Molecular analysis of single circulating tumour cells following long‐term storage of clinical samples
Sign Up to like & getrecommendations! Published in 2017 at "Molecular Oncology"
DOI: 10.1002/1878-0261.12113
Abstract: The CellSearch® semiautomated CTC enrichment and staining system has been established as the ‘gold standard’ for CTC enumeration with CellSearch® CTC counts recognized by the FDA as prognostic for a number of cancers. We and… read more here.
Keywords: storage; long term; cellsearch; molecular analysis ... See more keywords
Toward a Home Test for COVID‐19 Diagnosis: DNA Machine for Amplification‐Free SARS‐CoV‐2 Detection in Clinical Samples
Sign Up to like & getrecommendations! Published in 2022 at "Chemmedchem"
DOI: 10.1002/cmdc.202200382
Abstract: Nucleic acid‐based detection of RNA viruses requires an annealing procedure to obtain RNA/probe or RNA/primer complexes for unwinding stable structures of folded viral RNA. In this study, we designed a protein‐enzyme‐free nano‐construction, named four‐armed DNA… read more here.
Keywords: cov detection; rna; detection; clinical samples ... See more keywords
Diagnosis of Invasive Pulmonary Aspergillosis.
Sign Up to like & getrecommendations! Published in 2017 at "Advances in experimental medicine and biology"
DOI: 10.1007/5584_2016_46
Abstract: Culturing strains from clinical samples is the main method to diagnose invasive pulmonary aspergillosis. Detecting the galactomannan antigen in serum samples is an auxiliary examination. The goal of this study was to determine the frequency… read more here.
Keywords: fumigatus; galactomannan antigen; pulmonary aspergillosis; invasive pulmonary ... See more keywords
Whole-Genome Enrichment Using RNA Probes and Sequencing of Chlamydia trachomatis Directly from Clinical Samples.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7037-7_1
Abstract: Whole-genome sequencing is a powerful, high-resolution tool that can be used to generate accurate data on bacterial population structure, phylogeography, and mutations associated with antimicrobial resistance. The ability to sequence pathogen genomes directly from clinical… read more here.
Keywords: directly clinical; whole genome; chlamydia trachomatis; trachomatis ... See more keywords
Gordonia: isolation and identification in clinical samples and role in biotechnology
Sign Up to like & getrecommendations! Published in 2017 at "Folia Microbiologica"
DOI: 10.1007/s12223-017-0491-1
Abstract: Gordonia spp. are members of the actinomycete family, and the environment, especially soil, is the natural habitat of this genus of bacteria. Gordonia spp. are important for two aspects: first, some Gordonia species cause a… read more here.
Keywords: clinical samples; identification clinical; identification; isolation identification ... See more keywords
Distress intolerance mediates attentional control on posttraumatic stress symptoms: Evidence from two clinical samples.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of psychiatric research"
DOI: 10.1016/j.jpsychires.2020.06.018
Abstract: Theoretical models emphasize the importance of both affective and cognitive risk factors in the development of posttraumatic stress symptoms (PTSS). Two such factors predicting PTSS have been studied extensively: distress intolerance (DI) - an affective… read more here.
Keywords: posttraumatic stress; attentional control; stress symptoms; two clinical ... See more keywords
Molecular detection of feline calicivirus in clinical samples: A study comparing its detection by RT-qPCR directly from swabs and after virus isolation.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2017.10.001
Abstract: Feline caliciviruses (FCVs) are non-enveloped RNA viruses that exhibit high genetic variation. Two reverse transcription quantitative polymerase chain reaction (RT-qPCR) FCV assays (S1 and S2) were evaluated using samples from 300 field cats. The direct… read more here.
Keywords: detection; cell culture; molecular detection; feline ... See more keywords
Development of a quantitative real time RT-PCR assay for sensitive and rapid detection of emerging Atypical Porcine Pestivirus associated with congenital tremor in pigs.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of virological methods"
DOI: 10.1016/j.jviromet.2021.114220
Abstract: Atypical Porcine Pestivirus (APPV) is reported as the etiologic agent for type AII congenital tremors in newborn piglets. Initial PCR-based diagnostic tests to detect APPV were designed based on the limited sequence information and are… read more here.
Keywords: detection; porcine pestivirus; sensitivity; atypical porcine ... See more keywords
FastFung: A novel medium for the culture and isolation of fastidious fungal species from clinical samples.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of microbiological methods"
DOI: 10.1016/j.mimet.2020.106108
Abstract: We developed a novel culture medium, referred to FastFung medium as suitable for the culture of clinical fungi, including fastidious ones, for both research and diagnostic studies. It is based on Schædler agar supplemented with… read more here.
Keywords: fastfung medium; medium; samples fastfung; culture ... See more keywords
MINERVA: A Facile Strategy for SARS-CoV-2 Whole-Genome Deep Sequencing of Clinical Samples
Sign Up to like & getrecommendations! Published in 2020 at "Molecular Cell"
DOI: 10.1016/j.molcel.2020.11.030
Abstract: Analyzing the genome of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from clinical samples is crucial for the understanding of viral spread and viral evolution, as well as for vaccine development. Existing RNA sequencing methods… read more here.
Keywords: minerva facile; sequencing clinical; sars cov; facile strategy ... See more keywords
Seroprevalence and molecular characterization of Brucella species in naturally infected cattle and sheep.
Sign Up to like & getrecommendations! Published in 2019 at "Preventive veterinary medicine"
DOI: 10.1016/j.prevetmed.2019.104756
Abstract: Brucellosis is one of the most economically significant infectious diseases in Egypt. The study aimed to assess the seroprevalence by indirect ELISA by examining serum samples from 720 cattle and 320 sheep and detection and… read more here.
Keywords: cattle sheep; clinical samples; characterization brucella; seroprevalence ... See more keywords