High frequency of double crossover recombination facilitates genome engineering in Pseudomonas aeruginosa PA14 and clone C strains.
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DOI: 10.1099/mic.0.000812
Abstract: Pseudomonas aeruginosa is a key opportunistic human pathogen. An established procedure to replace a target gene is two-step allelic exchange, i.e. selection of single crossover at homologous sequences and subsequent counter selection to induce double… read more here.
Keywords: pseudomonas aeruginosa; frequency; crossover; double crossover ... See more keywords