Gene cloning, expression, and characterization of a novel β‐mannanase from the endophyte Paenibacillus sp. CH‐3
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.1510
Abstract: An endophyte with high yield of β‐mannanase, designated as Paenibacillus sp. CH‐3, was isolated from black locust (Robinia pseudoacacia) seeds. The β‐mannanase gene (manB) has been cloned, expressed, purified, and characterized. With an open reading… read more here.
Keywords: expression characterization; mannanase; manb; gene cloning ... See more keywords
Cloning, expression and characterization of a novel sericin-like protein.
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology and applied biochemistry"
DOI: 10.1002/bab.2089
Abstract: Silk consists of two proteins called fibroin and sericin. While fibroin is used in the textile industry and has various biomaterial applications, sericin has been considered as waste material until recently. Sericin is a multi-component… read more here.
Keywords: protein; sericin like; sericin; like protein ... See more keywords
Gene cloning and expression of a glucoside 3-dehydrogenase from Sphingobacterium faecium ZJF-D6, and used it to produce N-p-nitrophenyl-3-ketovalidamine
Sign Up to like & getrecommendations! Published in 2017 at "World Journal of Microbiology and Biotechnology"
DOI: 10.1007/s11274-016-2187-0
Abstract: In this study, we report the cloning and expression of a functional glucoside 3-dehydrogenase (G3DH) gene from Sphingobacterium faecium ZJF-D6. This gene is 1686 bp in length and encodes a peptide of 562 amino acids. The… read more here.
Keywords: faecium zjf; nitrophenyl ketovalidamine; sphingobacterium faecium; glucoside dehydrogenase ... See more keywords
Cloning, Expression and Characterization of a Highly Active Alcohol Dehydrogenase for Production of Ethyl (S)-4-Chloro-3-Hydroxybutyrate
Sign Up to like & getrecommendations! Published in 2019 at "Indian Journal of Microbiology"
DOI: 10.1007/s12088-019-00795-0
Abstract: A novel alcohol dehydrogenase from Bartonella apis (BaADH) was heterologous expressed in Escherichia coli. Its biochemical properties were investigated and used to catalyze the synthesis of ethyl (S)-4-chloro-3-hydroxybutanoate ((S)-CHBE), which is a chiral intermediate of… read more here.
Keywords: ethyl chloro; dehydrogenase; cloning expression; alcohol dehydrogenase ... See more keywords
Cloning and expression studies on glutathione S-transferase like-gene in honey bee for its role in oxidative stress
Sign Up to like & getrecommendations! Published in 2022 at "Cell Stress and Chaperones"
DOI: 10.1007/s12192-022-01255-3
Abstract: Glutathione S-transferases (GSTs) constitute an important multifunctional enzyme family that plays vital roles in cellular detoxification and protecting organisms against oxidative stress caused by reactive oxygen species (ROS). In this study, we isolated a GST-… read more here.
Keywords: stress; like gene; cloning expression; oxidative stress ... See more keywords
Cloning, Expression, and Characterization of a Thermotolerant β-agarase from Simiduia sp. SH-4
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioprocess Engineering"
DOI: 10.1007/s12257-018-0072-4
Abstract: The gene coding for a thermotolerant β-agarase from an isolated Simiduia sp. SH-4 was cloned, recombinantly expressed, and characterized after purification. This gene was sequenced after cassette mediated polymerase chain reaction and composed of an… read more here.
Keywords: agarase; expression characterization; agarase simiduia; thermotolerant agarase ... See more keywords
Gene cloning, expression and homology modeling of first fibrinolytic enzyme from mushroom (Cordyceps militaris).
Sign Up to like & getrecommendations! Published in 2019 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2019.09.212
Abstract: Fibrinolytic enzymes are important thrombolytic agents for blood-clotting disorders like cardiovascular diseases. Availability of novel recombinant fibrinolytic enzymes can overcome the shortcomings of current thrombolytic drugs. With the objective of facilitating their cost-effective production for… read more here.
Keywords: cordyceps militaris; fibrinolytic enzyme; enzyme; cloning expression ... See more keywords
Cloning, expression, and characterization of a novel superoxide dismutase from deep-sea sea cucumber.
Sign Up to like & getrecommendations! Published in 2020 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2020.09.135
Abstract: Superoxide dismutases (SODs) are the first line of defense against oxidative damage caused by reactive oxygen species and are associated with stress tolerance. Accordingly, many studies have focused on SODs, especially those from extreme habitats.… read more here.
Keywords: characterization novel; deep sea; expression characterization; sea ... See more keywords
Cloning and expression of pullulanase from Bacillus subtilis BK07 and PY22 in Pichia pastoris.
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DOI: 10.1016/j.pep.2019.05.008
Abstract: In this study, pullulanase genes from a wild isolate B. subtilis BK07 and B. subtilis PY22 (mutant strain derived from B. subtilis 168) were transformed into P. pastoris KM71H. Extracellular recombinant protein production was achieved with methanol induction under… read more here.
Keywords: expression pullulanase; activity; cloning expression; subtilis bk07 ... See more keywords
Phage N15-Based Vectors for Gene Cloning and Expression in Bacteria and Mammalian Cells.
Sign Up to like & getrecommendations! Published in 2023 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00580
Abstract: Bacteriophage N15 is the first virus known to deliver linear prophage into Escherichia coli. During its lysogenic cycle, N15 protelomerase (TelN) resolves its telomerase occupancy site (tos) into hairpin telomeres. This protects the N15 prophage… read more here.
Keywords: n15 based; mammalian cells; cloning expression; gene ... See more keywords
Cloning and expression of a recombinant tumor necrosis factor-α (TNF-α) protein
Sign Up to like & getrecommendations! Published in 2019 at "Annals of Medicine"
DOI: 10.1080/07853890.2018.1561799
Abstract: Introduction: Rheumatoid arthritis (RA) is a chronic systemic inflammatory disease and in many patients can lead to joint destruction and loss of function. The expression of the proinflammatory cytokine (TNF-a) is widely related to the… read more here.
Keywords: protein; expression; rtnf; tnf protein ... See more keywords