Articles with "cloning functional" as a keyword



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Molecular cloning and functional analysis of a biphenyl phytoalexin-specific O-methyltransferase from apple cell suspension cultures

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Published in 2018 at "Planta"

DOI: 10.1007/s00425-018-3031-6

Abstract: Main conclusionThis manuscript describes the cloning and functional characterization of a biphenyl phytoalexin biosynthetic gene, 3,5-dihydroxybiphenyl O-methyltransferase from elicitor-treated cell cultures of scab resistant apple cultivar ‘Florina’.Apples belong to the subtribe Malinae of the Rosaceae… read more here.

Keywords: methyltransferase; biphenyl; cloning functional; apple ... See more keywords
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Molecular cloning and functional characterization of a sweetpotato chloroplast IbDHAR3 gene in response to abiotic stress

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Published in 2019 at "Plant Biotechnology Reports"

DOI: 10.1007/s11816-019-00576-7

Abstract: Dehydroascorbate reductase (DHAR) plays a critical role in the regeneration of ascorbic acid (AsA), and widely involved in plant tolerance to biotic and abiotic stresses. In this study, the IbDHAR3 gene was cloned from sweetpotato… read more here.

Keywords: molecular cloning; functional characterization; chloroplast; cloning functional ... See more keywords
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Molecular cloning and functional characterization of elongase (elovl5) and fatty acyl desaturase (fads2) in sciaenid, Nibea diacanthus (Lacepède, 1802).

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Published in 2019 at "Gene"

DOI: 10.1016/j.gene.2019.01.033

Abstract: In the present paper, we investigated the molecular cloning and functional characterization of elongase of very long chain fatty acid (elovl) and fatty acyl desaturase (fads) genes in a marine teleost, Nibea diacanthus. The elongase… read more here.

Keywords: molecular cloning; desaturase; elongase; functional characterization ... See more keywords
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Cloning and functional analysis of AmDUF1517 promoter from Ammopiptanthus mongolicus.

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Published in 2020 at "Journal of bioscience and bioengineering"

DOI: 10.1016/j.jbiosc.2020.04.014

Abstract: Domains of unknown function protein family 1517 (DUF1517) in Ammopiptanthus mongolicus, could be induced by abiotic stresses, whose upstream regulatory sequence might be an ideal source of abiotic-induced promoter. In this study, a 1026-bp promoter… read more here.

Keywords: analysis; amduf1517 promoter; cloning functional; promoter ... See more keywords
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Molecular cloning and functional characterization of an isoflavone glucosyltransferase from Pueraria thomsonii.

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Published in 2022 at "Chinese journal of natural medicines"

DOI: 10.1016/s1875-5364(21)60105-x

Abstract: Pueraria thomsonii has long been used in traditional Chinese medicine. Isoflavonoids are the principle pharmacologically active components, which are primarily observed as glycosyl-conjugates and accumulate in P. thomsonii roots. However, the molecular mechanisms underlying the… read more here.

Keywords: glucosyltransferase; pueraria thomsonii; cloning functional; functional characterization ... See more keywords
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Cloning, functional characterization and screening of potential inhibitors for Chilo partellus chitin synthase A using in silico, in vitro and in vivo approaches

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Published in 2020 at "Journal of Biomolecular Structure and Dynamics"

DOI: 10.1080/07391102.2020.1827034

Abstract: Abstract Chitin synthase (CHS) is one of the crucial enzymes that play an essential role in chitin synthesis during the molting process, and it is considered to be the specific target to control insect pests.… read more here.

Keywords: vitro vivo; chitin; cloning functional; chitin synthase ... See more keywords
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Molecular cloning and functional analysis of MbWRKY3 involved in improved drought tolerance in transformed tobacco

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Published in 2018 at "Journal of Plant Interactions"

DOI: 10.1080/17429145.2018.1478994

Abstract: ABSTRACT Plant-specific WRKY transcription factors were involved in stress responses and ABA signaling. In the present study, a WRKY gene is isolated from Malus baccata (L.) Borkh and designated as MbWRKY3. Subcellular localization revealed that… read more here.

Keywords: molecular cloning; tolerance; cloning functional; mbwrky3 ... See more keywords
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Cloning and functional identification of moricins from the diamondback moth, Plutella xylostella (L.)

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Published in 2017 at "Insect Molecular Biology"

DOI: 10.1111/imb.12319

Abstract: Antimicrobial peptides (AMPs) are small‐molecule peptides that play crucial roles in insect innate immune responses. To better understand the function of AMPs in Plutella xylostella, one of the main pests of cruciferous vegetables, three full‐length… read more here.

Keywords: microscopy; identification moricins; plutella xylostella; cloning functional ... See more keywords
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Cloning and Functional Characterization of CsUGD2 in Cucumber (Cucumis sativus L.)

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Published in 2018 at "Notulae Botanicae Horti Agrobotanici Cluj-Napoca"

DOI: 10.15835/nbha47111289

Abstract: UGD gene encodes UDP-glucose dehydrogenase (UGD) which is a key enzyme in the biosynthesis of cell wall, and it catalyses the irreversible oxidation of UDP-glucose (UDP-Glc) into UDP-glucuronic acid (UDP-GlcA). In cucumber, the expression level… read more here.

Keywords: cucumber; functional characterization; csugd2; cloning functional ... See more keywords
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Corrigendum: Cloning and functional validation of early inducible Magnaporthe oryzae responsive CYP76M7 promoter from rice

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Published in 2018 at "Frontiers in Plant Science"

DOI: 10.3389/fpls.2018.00939

Abstract: [This corrects the article on p. 371 in vol. 6, PMID: 26052337.]. read more here.

Keywords: validation early; functional validation; corrigendum cloning; cloning functional ... See more keywords
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Molecular Cloning and Functional Analysis of the NPR1 Homolog in Kiwifruit (Actinidia eriantha)

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Published in 2020 at "Frontiers in Plant Science"

DOI: 10.3389/fpls.2020.551201

Abstract: Kiwifruit bacterial canker, caused by the bacterial pathogen Pseudomonas syringae pv. actinidiae (Psa), is a destructive disease in the kiwifruit industry globally. Consequently, understanding the mechanism of defense against pathogens in kiwifruit could facilitate the… read more here.

Keywords: molecular cloning; npr1; analysis; cloning functional ... See more keywords