Overview of High-Throughput Cloning Methods for the Post-genomic Era.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9624-7_1
Abstract: The advent of new DNA sequencing technologies leads to a dramatic increase in the number of available genome sequences and therefore of target genes with potential for functional analysis. The insertion of these sequences into… read more here.
Keywords: throughput; cloning methods; methods post; throughput cloning ... See more keywords
Evaluation of the efficiency and utility of recombinant enzyme-free seamless DNA cloning methods
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DOI: 10.1016/j.bbrep.2017.01.010
Abstract: Simple and low-cost recombinant enzyme-free seamless DNA cloning methods have recently become available. In vivo Escherichia coli cloning (iVEC) can directly transform a mixture of insert and vector DNA fragments into E. coli, which are… read more here.
Keywords: dna; cloning methods; seamless; seamless dna ... See more keywords
A User’s Guide to Golden Gate Cloning Methods and Standards
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DOI: 10.1021/acssynbio.2c00355
Abstract: The continual demand for specialized molecular cloning techniques that suit a broad range of applications has driven the development of many different cloning strategies. One method that has gained significant traction is Golden Gate assembly,… read more here.
Keywords: golden gate; guide golden; cloning methods; user guide ... See more keywords