An efficient method for markerless mutant generation by allelic exchange in Clostridium acetobutylicum and Clostridium saccharobutylicum using suicide vectors
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DOI: 10.1186/s13068-019-1364-4
Abstract: BackgroundClostridium acetobutylicum and Clostridium saccharobutylicum are Gram-positive, spore-forming, anaerobic bacterium capable of converting various sugars and polysaccharides into solvents (acetone, butanol, and ethanol). The sequencing of their genomes has prompted new approaches to genetic analysis,… read more here.
Keywords: suicide vectors; clostridium saccharobutylicum; method; clostridium ... See more keywords