Engineering cellulolytic bacterium Clostridium thermocellum to co‐ferment cellulose‐ and hemicellulose‐derived sugars simultaneously
Sign Up to like & getrecommendations! Published in 2018 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.26590
Abstract: Cellulose and hemicellulose are the most abundant components in plant biomass. A preferred Consolidated Bioprocessing (CBP) system is one which can directly convert both cellulose and hemicellulose into target products without adding the costly hydrolytic… read more here.
Keywords: clostridium thermocellum; bacterium clostridium; cellulose; cellulose hemicellulose ... See more keywords
Evaluation of promoter sequences for the secretory production of a Clostridium thermocellum cellulase in Paenibacillus polymyxa
Sign Up to like & getrecommendations! Published in 2018 at "Applied Microbiology and Biotechnology"
DOI: 10.1007/s00253-018-9369-7
Abstract: Due to their high secretion capacity, Gram-positive bacteria from the genus Bacillus are important expression hosts for the high-yield production of enzymes in industrial biotechnology; however, to date, strains from only few Bacillus species are… read more here.
Keywords: production; promoter sequences; expression; promoter ... See more keywords
Resonance assignments of a cellulosomal double-dockerin from Clostridium thermocellum
Sign Up to like & getrecommendations! Published in 2019 at "Biomolecular NMR Assignments"
DOI: 10.1007/s12104-018-9859-7
Abstract: Cellulosomes are highly efficient multienzyme complexes for lignocellulose degradation secreted by some lignocellulolytic bacteria. Cellulosomes are assembled through protein modules named cohesin and dockerin, and multiple cohesin modules in the scaffold protein generally determine the… read more here.
Keywords: clostridium thermocellum; multiple dockerin; double dockerin; dockerin ... See more keywords
Novel insights into the degradation of β-1,3-glucans by the cellulosome of Clostridium thermocellum revealed by structure and function studies of a family 81 glycoside hydrolase.
Sign Up to like & getrecommendations! Published in 2018 at "International journal of biological macromolecules"
DOI: 10.1016/j.ijbiomac.2018.06.003
Abstract: The family 81 glycoside hydrolase (GH81) from Clostridium thermocellum is a β-1,3-glucanase belonging to cellulosomal complex. The gene encoding GH81 from Clostridium thermocellum (CtLam81A) was cloned and expressed displaying a molecular mass of ~82 kDa. CtLam81A… read more here.
Keywords: family glycoside; glycoside hydrolase; ctlam81a; clostridium ... See more keywords
In vivo evolution of lactic acid hyper-tolerant Clostridium thermocellum.
Sign Up to like & getrecommendations! Published in 2021 at "New biotechnology"
DOI: 10.1016/j.nbt.2021.12.003
Abstract: Lactic acid (LA) has several applications in the food, cosmetics and pharmaceutical industries, as well as in the production of biodegradable plastic polymers, namely polylactides. Industrial production of LA is essentially based on microbial fermentation.… read more here.
Keywords: production; lactic acid; clostridium thermocellum; thermocellum ... See more keywords
Glycolysis without pyruvate kinase in Clostridium thermocellum.
Sign Up to like & getrecommendations! Published in 2017 at "Metabolic engineering"
DOI: 10.1016/j.ymben.2016.11.011
Abstract: The metabolism of Clostridium thermocellum is notable in that it assimilates sugar via the EMP pathway but does not possess a pyruvate kinase enzyme. In the wild type organism, there are three proposed pathways for… read more here.
Keywords: clostridium thermocellum; pyruvate kinase; shunt; malate shunt ... See more keywords
Engineering a spermidine biosynthetic pathway in Clostridium thermocellum results in increased resistance to furans and increased ethanol production.
Sign Up to like & getrecommendations! Published in 2018 at "Metabolic engineering"
DOI: 10.1016/j.ymben.2018.09.002
Abstract: Polyamines are low molecular weight aliphatic nitrogen compounds found ubiquitously in microorganisms, plants, and animals. Spermidine is a common polyamine that plays a role in stabilizing chromatin, DNA replication, transcription, translation, as well as the… read more here.
Keywords: tolerance; engineering; clostridium thermocellum; spermidine ... See more keywords
Deciphering Cellodextrin and Glucose Uptake in Clostridium thermocellum
Sign Up to like & getrecommendations! Published in 2022 at "mBio"
DOI: 10.1128/mbio.01476-22
Abstract: Highly efficient sugar uptake is important to microbial cell factories, and sugar transporters are therefore of great interest in the study of industrially relevant microorganisms. Clostridium thermocellum is a lignocellulolytic bacterium known for its multienzyme… read more here.
Keywords: sugar; transporter; clostridium thermocellum; bacterium ... See more keywords
Expression of a heat-stable NADPH-dependent alcohol dehydrogenase from Thermoanaerobacter pseudethanolicus 39E in Clostridium thermocellum 1313 results in increased hydroxymethylfurfural resistance
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology for Biofuels"
DOI: 10.1186/s13068-017-0750-z
Abstract: BackgroundResistance to deconstruction is a major limitation to the use of lignocellulosic biomass as a substrate for the production of fuels and chemicals. Consolidated bioprocessing (CBP), the use of microbes for the simultaneous hydrolysis of… read more here.
Keywords: thermoanaerobacter pseudethanolicus; pseudethanolicus 39e; thermocellum; expression ... See more keywords
Consolidated bioprocess for bioethanol production from lignocellulosic biomass using Clostridium thermocellum DSM 1237
Sign Up to like & getrecommendations! Published in 2020 at "Bioresources"
DOI: 10.15376/biores.15.4.8355-8368
Abstract: A consolidated bioprocessing (CBP) using Clostridium thermocellum DSM 1237 for bioethanol production in anaerobic bottles and a 3-L fermenter from biomass was investigated. The effects of key operational parameters including different carbon sources, temperature, and… read more here.
Keywords: clostridium thermocellum; bioethanol production; bioethanol; biomass ... See more keywords
Expression of serpins by Clostridium thermocellum and simultaneous saccharification of lignocellulosic biomass to enhance ethanol production
Sign Up to like & getrecommendations! Published in 2020 at "Bioresources"
DOI: 10.15376/biores.15.4.8662-8676
Abstract: Utilization of biomass for production of second generation bioethanol was considered as a way to reduce burdens of fossil fuel in Pakistan. The materials wheat straw, rice straw, cotton stalk, corn stover, and peel wastes… read more here.
Keywords: production; biomass; wheat straw; clostridium thermocellum ... See more keywords