High doses of CRISPR/Cas9 ribonucleoprotein efficiently induce gene knockout with low mosaicism in the hydrozoan Clytia hemisphaerica through microhomology-mediated deletion
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DOI: 10.1038/s41598-018-30188-0
Abstract: Targeted mutagenesis using CRISPR/Cas9 technology has been shown to be a powerful approach to examine gene function in diverse metazoan species. One common drawback is that mixed genotypes, and thus variable phenotypes, arise in the… read more here.
Keywords: microhomology mediated; clytia; crispr cas9; gene knockout ... See more keywords