Purification of Clathrin-Coated Vesicles from Adult Rat Brain.
Sign Up to like & getrecommendations! Published in 2018 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-8719-1_1
Abstract: Here, we describe a purification protocol for isolating clathrin-coated vesicles (CCVs) from adult rat brain by using differential centrifugation coupled with Ficoll-sucrose and D2O-sucrose density gradient centrifugation and an additional linear sucrose step gradient at… read more here.
Keywords: adult rat; purification; clathrin coated; rat brain ... See more keywords
Architecture of the AP2/clathrin coat on the membranes of clathrin-coated vesicles
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DOI: 10.1126/sciadv.aba8381
Abstract: Cryo-electron tomography reveals the conformational changes and clathrin interactions of AP2 during coated vesicle assembly. Clathrin-mediated endocytosis (CME) is crucial for modulating the protein composition of a cell’s plasma membrane. Clathrin forms a cage-like, polyhedral… read more here.
Keywords: architecture ap2; clathrin coated; coat; ap2 clathrin ... See more keywords
The structures of natively assembled clathrin-coated vesicles
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DOI: 10.1126/sciadv.aba8397
Abstract: Distribution of adaptor proteins in clathrin-coated vesicles is not random. Clathrin-coated vesicles mediate trafficking of proteins and nutrients in the cell and between organelles. Proteins included in the clathrin-coated vesicles (CCVs) category include clathrin heavy… read more here.
Keywords: hexagonal faces; clathrin coated; structures natively; coated vesicles ... See more keywords
Preparation of Clathrin-Coated Vesicles From Arabidopsis thaliana Seedlings
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DOI: 10.3389/fpls.2018.01972
Abstract: Clathrin coated vesicles (CCVs) mediate endocytosis of plasma membrane proteins and deliver their content to the endosomes for either subsequent recycling to the plasma membrane or transport to the vacuole for degradation. CCVs assemble also… read more here.
Keywords: ccvs; thaliana seedlings; arabidopsis thaliana; clathrin coated ... See more keywords