Programmed ubiquitin acetylation using genetic code expansion reveals altered ubiquitination patterns
Sign Up to like & getrecommendations! Published in 2019 at "FEBS Letters"
DOI: 10.1002/1873-3468.13702
Abstract: Ubiquitination is a post‐translational modification (PTM) capable of being regulated by other PTMs, including acetylation. However, the biological consequences of acetylated ubiquitin (acUb) variants are poorly understood, due to their transient nature in vivo and… read more here.
Keywords: code expansion; acetylation; ubiquitination patterns; using genetic ... See more keywords
Expanding the Genetic Code for Neuronal Studies
Sign Up to like & getrecommendations! Published in 2020 at "Chembiochem"
DOI: 10.1002/cbic.202000300
Abstract: Genetic code expansion is one of the most powerful technologies in protein engineering. In addition to the 20 canonical amino acids, the expanded genetic code is supplemented by unnatural amino acids, which have artificial side… read more here.
Keywords: code expansion; amino acids; code; code neuronal ... See more keywords
Genetic code expansion via integration of redundant amino acid assignment by finely tuning tRNA pools.
Sign Up to like & getrecommendations! Published in 2018 at "Current opinion in chemical biology"
DOI: 10.1016/j.cbpa.2018.07.010
Abstract: In all translation systems, the genetic code assigns codons to amino acids as building blocks of polypeptides, defining their chemical, structural and physiological properties. The canonical genetic code, however, utilizes only 20 proteinogenic amino acids… read more here.
Keywords: code expansion; amino acids; code; expansion via ... See more keywords
New opportunities for genetic code expansion in synthetic yeast.
Sign Up to like & getrecommendations! Published in 2022 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2022.102691
Abstract: The synthetic yeast, Sc2.0, is nearing completion as consolidation of all 17 synthetic chromosomes into a single cell advances. This organism will be the first synthetic eukaryote and provides a highly plastic biological chassis built… read more here.
Keywords: code expansion; yeast; synthetic yeast; genetic code ... See more keywords
Engineering translation components for genetic code expansion.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of molecular biology"
DOI: 10.1016/j.jmb.2021.167302
Abstract: The expansion of the genetic code consisting of four bases and 20 amino acids into diverse building blocks has been an exciting topic in synthetic biology. Many biochemical components are involved in gene expression; therefore,… read more here.
Keywords: code expansion; engineering; code; biology ... See more keywords
Deciphering the Interactome of Histone Marks in Living Cells via Genetic Code Expansion Combined with Proximity Labeling.
Sign Up to like & getrecommendations! Published in 2022 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.2c01042
Abstract: Deciphering the endogenous interactors of histone post-translational modifications (hPTMs, also called histone marks) is essential to understand the mechanisms of epigenetic regulation. However, most of the analytical methods to determine hPTM interactomes are in vitro… read more here.
Keywords: histone marks; histone; genetic code; code expansion ... See more keywords
Genetic Code Expansion: Recent Developments and Emerging Applications
Sign Up to like & getrecommendations! Published in 2024 at "Chemical Reviews"
DOI: 10.1021/acs.chemrev.4c00216
Abstract: The concept of genetic code expansion (GCE) has revolutionized the field of chemical and synthetic biology, enabling the site-specific incorporation of noncanonical amino acids (ncAAs) into proteins, thus opening new avenues in research and applications… read more here.
Keywords: medicine; genetic code; biology; code expansion ... See more keywords
Engineering Pyrrolysine Systems for Genetic Code Expansion and Reprogramming
Sign Up to like & getrecommendations! Published in 2024 at "Chemical Reviews"
DOI: 10.1021/acs.chemrev.4c00243
Abstract: Over the past 16 years, genetic code expansion and reprogramming in living organisms has been transformed by advances that leverage the unique properties of pyrrolysyl-tRNA synthetase (PylRS)/tRNAPyl pairs. Here we summarize the discovery of the… read more here.
Keywords: trnapyl pairs; genetic code; code expansion; pylrs trnapyl ... See more keywords
Genetic Code Expansion for Mechanistic Studies in Ion Channels: An (Un)natural Union of Chemistry and Biology
Sign Up to like & getrecommendations! Published in 2024 at "Chemical Reviews"
DOI: 10.1021/acs.chemrev.4c00306
Abstract: Ion channels play central roles in biology and human health by catalyzing the transmembrane flow of electrical charge. These proteins are ideal targets for genetic code expansion (GCE) methods because it is feasible to measure… read more here.
Keywords: chemistry; code expansion; ion channels; biology ... See more keywords
Genetic Code Expansion: Inception, Development, Commercialization.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.0c11938
Abstract: Virtually all natural proteins are built from only 20 amino acids, and while this makes possible all the functions they perform, the ability to encode other amino acids selected for specific purposes promises to enable… read more here.
Keywords: expansion inception; commercialization; genetic code; code expansion ... See more keywords
An orthogonalized platform for genetic code expansion in both bacteria and eukaryotes.
Sign Up to like & getrecommendations! Published in 2017 at "Nature chemical biology"
DOI: 10.1038/nchembio.2312
Abstract: In this study, we demonstrate the feasibility of expanding the genetic code of Escherichia coli using its own tryptophanyl-tRNA synthetase and tRNA (TrpRS-tRNATrp) pair. This was made possible by first functionally replacing this endogenous pair… read more here.
Keywords: trprs trnatrp; code expansion; platform genetic; code ... See more keywords