Optimized expression of the Starmerella bombicola lactone esterase in Pichia pastoris through temperature adaptation, codon-optimization and co-expression with HAC1.
Sign Up to like & getrecommendations! Published in 2018 at "Protein expression and purification"
DOI: 10.1016/j.pep.2017.10.016
Abstract: The Starmerella bombicola lactone esterase (SBLE) is a novel enzyme that, in vivo, catalyzes the intramolecular esterification (lactonization) of acidic sophorolipids in an aqueous environment. In fact, this is an unusual reaction given the unfavorable… read more here.
Keywords: starmerella bombicola; lactone esterase; expression; codon optimization ... See more keywords
"CodonWizard" - An intuitive software tool with graphical user interface for customizable codon optimization in protein expression efforts.
Sign Up to like & getrecommendations! Published in 2019 at "Protein expression and purification"
DOI: 10.1016/j.pep.2019.03.018
Abstract: Optimization of coding sequences to maximize protein expression yield is often outsourced to external service providers during commercial gene synthesis and thus unfortunately remains a black box for many researchers. The presented software program "CodonWizard"… read more here.
Keywords: protein expression; graphical user; customizable codon; optimization ... See more keywords
Optimizing assembly and production of native bispecific antibodies by codon de-optimization
Sign Up to like & getrecommendations! Published in 2017 at "mAbs"
DOI: 10.1080/19420862.2016.1267088
Abstract: ABSTRACT When production of bispecific antibodies requires the co-expression and assembly of three or four polypeptide chains, low expression of one chain can significantly limit assembly and yield. κλ bodies, fully human bispecific antibodies with… read more here.
Keywords: chain; bispecific antibodies; expression; codon optimization ... See more keywords
Cloning and codon optimization of a novel feline interferon omega gene for production by Pichia pastoris and its antiviral efficacy in polyethylene glycol-modified form
Sign Up to like & getrecommendations! Published in 2022 at "Virulence"
DOI: 10.1080/21505594.2022.2029330
Abstract: ABSTRACT Feline viral diseases, such as feline panleukopenia, feline infectious peritonitis, and feline coronaviral enteritis, seriously endanger the health of cats, and restrict the development of pet industry. Meanwhile, there is a current lack of… read more here.
Keywords: antiviral efficacy; pichia pastoris; codon optimization; polyethylene glycol ... See more keywords
mRNA codon optimization with quantum computers
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DOI: 10.1371/journal.pone.0259101
Abstract: Reverse translation of polypeptide sequences to expressible mRNA constructs is a NP-hard combinatorial optimization problem. Each amino acid in the protein sequence can be represented by as many as six codons, and the process of… read more here.
Keywords: quantum computers; mrna codon; optimization quantum; optimization ... See more keywords
Validation of Promoters and Codon Optimization on CRISPR/Cas9-Engineered Jurkat Cells Stably Expressing αRep4E3 for Interfering with HIV-1 Replication
Sign Up to like & getrecommendations! Published in 2022 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms232315049
Abstract: Persistent and efficient therapeutic protein expression in the specific target cell is a significant concern in gene therapy. The controllable integration site, suitable promoter, and proper codon usage influence the effectiveness of the therapeutic outcome.… read more here.
Keywords: jurkat cells; crispr cas9; codon optimization; jurkat ... See more keywords
Codon optimization underpins generalist parasitism in fungi
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DOI: 10.7554/elife.22472
Abstract: The range of hosts that parasites can infect is a key determinant of the emergence and spread of disease. Yet, the impact of host range variation on the evolution of parasite genomes remains unknown. Here,… read more here.
Keywords: host range; generalist parasitism; optimization; codon optimization ... See more keywords