Articles with "codon optimized" as a keyword



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Enhanced 5-Aminolevulinic Acid Production by Co-expression of Codon-Optimized hemA Gene with Chaperone in Genetic Engineered Escherichia coli

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Published in 2019 at "Applied Biochemistry and Biotechnology"

DOI: 10.1007/s12010-019-03178-9

Abstract: 5-Aminolevulinic acid (ALA) is an important metabolic intermediate compound with high value and has recently been used in agriculture and medicine. In this study, we have constructed six recombinant Escherichia coli (E. coli) strains that… read more here.

Keywords: codon optimized; production; escherichia coli; aminolevulinic acid ... See more keywords
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Efficient Coproduction of Mannanase and Cellulase by the Transformation of a Codon-Optimized Endomannanase Gene from Aspergillus niger into Trichoderma reesei.

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Published in 2017 at "Journal of agricultural and food chemistry"

DOI: 10.1021/acs.jafc.7b05114

Abstract: Cellulase and mannanase are both important enzyme additives in animal feeds. Expressing the two enzymes simultaneously within one microbial host could potentially lead to cost reductions in the feeding of animals. For this purpose, we… read more here.

Keywords: aspergillus niger; codon optimized; trichoderma reesei; cellulase ... See more keywords
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Expression and characterization of a codon‐optimized blood coagulation factor VIII

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Published in 2017 at "Journal of Thrombosis and Haemostasis"

DOI: 10.1111/jth.13632

Abstract: Essentials Recombinant factor VIII (FVIII) is known to be expressed at a low level in cell culture. To increase expression, we used codon‐optimization of a B‐domain deleted FVIII (BDD‐FVIII). This resulted in 7‐fold increase of… read more here.

Keywords: codon optimized; expression characterization; factor viii; characterization codon ... See more keywords