Regiospecific Coelenterazine Analogs for Bioassays and Molecular Imaging.
Sign Up to like & getrecommendations! Published in 2024 at "Bioconjugate chemistry"
DOI: 10.1021/acs.bioconjchem.4c00303
Abstract: Bioluminescence (BL) generated by luciferase-coelenterazine (CTZ) reactions is broadly employed as an optical readout in bioassays and in vivo molecular imaging. In this study, we demonstrate a systematic approach to elucidate the luciferase-CTZ binding chemistry… read more here.
Keywords: chemistry; regioisomeric ctz; coelenterazine; molecular imaging ... See more keywords
Insights into the bioluminescence systems of three sea pens (Cnidaria: Anthozoa): from de novo transcriptome analyses to biochemical assays
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DOI: 10.1098/rsob.240262
Abstract: Bioluminescence is the production of visible light by living organisms. It occurs through the oxidation of a luciferin substrate catalysed by luciferase enzymes. Auxiliary proteins, such as fluorescent proteins and luciferin-binding proteins, can modify the… read more here.
Keywords: transcriptome analyses; coelenterazine; three sea; binding proteins ... See more keywords
Chiral deaza-coelenterazine analogs for probing a substrate-binding site in the Ca2+-binding photoprotein aequorin
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DOI: 10.1371/journal.pone.0251743
Abstract: The Ca2+-binding photoprotein aequorin is a complex of apoAequorin (apoprotein) and (S)-2-peroxycoelenterazine. Aequorin can be regenerated by the incubation of apoAequorin with coelenterazine and molecular oxygen (O2). In this study, to investigate the molecular recognition… read more here.
Keywords: binding photoprotein; chiral deaza; coelenterazine; ca2 binding ... See more keywords