Cofactor recycling strategies for secondary metabolite production in cell-free protein expression systems
Sign Up to like & getrecommendations! Published in 2024 at "Biophysical Reviews"
DOI: 10.1007/s12551-024-01234-1
Abstract: Cell-free protein synthesis (CFPS) has emerged as an attractive platform for biotechnology and synthetic biology due to its numerous advantages to cell-based technologies for specific applications. CFPS can be faster, less sensitive to metabolite toxicity,… read more here.
Keywords: metabolite production; cell free; secondary metabolite; cofactor recycling ... See more keywords
Enzyme Assembly on Nanoparticle Scaffolds Enhances Cofactor Recycling and Improves Coupled Reaction Kinetics
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DOI: 10.1039/d3nr00729d
Abstract: Enzyme activity can be many times enhanced in configurations where they are displayed on a nanoparticle (NP) and this same format sometimes even provides access to channeling phenomena within multienzyme cascades. Here, we demonstrate that… read more here.
Keywords: nanoparticle scaffolds; cofactor recycling; enzyme assembly; cofactor ... See more keywords
H2-driven xylitol production in Cupriavidus necator H16
Sign Up to like & getrecommendations! Published in 2024 at "Microbial Cell Factories"
DOI: 10.1186/s12934-024-02615-7
Abstract: Background Biocatalysis offers a potentially greener alternative to chemical processes. For biocatalytic systems requiring cofactor recycling, hydrogen emerges as an attractive reducing agent. Hydrogen is attractive because all the electrons can be fully transferred to… read more here.
Keywords: cupriavidus necator; necator h16; necator; xylitol ... See more keywords