Switching the Cofactor Specificity of an Imine Reductase
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DOI: 10.1002/cctc.201701194
Abstract: In the last years, imine reductases (IREDs) have gained importance for the formation of chiral amines by catalyzing asymmetric reductions of imines and chemo‐ and stereoselective reductive aminations. However, all characterized members of this steadily… read more here.
Keywords: imine reductase; cofactor specificity; switching cofactor; specificity imine ... See more keywords
Enzyme Nicotinamide Cofactor Specificity Reversal Guided by Automated Structural Analysis and Library Design.
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DOI: 10.1007/978-1-4939-7295-1_2
Abstract: The specificity of enzymes for nicotinamide adenine dinucleotide (NAD) or nicotinamide adenine dinucleotide phosphate (NADP) as redox carriers can pose a significant hurdle for metabolic engineering and synthetic biology applications, where switching the specificity might… read more here.
Keywords: enzyme nicotinamide; cofactor specificity; biology; nicotinamide cofactor ... See more keywords
Logistic Regression-Guided Identification of Cofactor Specificity-Contributing Residues in Enzyme with Sequence Datasets Partitioned by Catalytic Properties
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DOI: 10.1021/acssynbio.2c00315
Abstract: Changing the substrate/cofactor specificity of an enzyme requires multiple mutations at spatially adjacent positions around the substrate pocket. However, this is challenging when solely based on crystal structure information because enzymes undergo dynamic conformational changes… read more here.
Keywords: logistic regression; cofactor specificity; cofactor; specificity ... See more keywords
An evolutionary path to altered cofactor specificity in a metalloenzyme
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DOI: 10.1038/s41467-020-16478-0
Abstract: Almost half of all enzymes utilize a metal cofactor. However, the features that dictate the metal utilized by metalloenzymes are poorly understood, limiting our ability to manipulate these enzymes for industrial and health-associated applications. The… read more here.
Keywords: cofactor specificity; evolutionary path; metal; metal specificity ... See more keywords