Biosynthesis of the human milk oligosaccharide 3‐fucosyllactose in metabolically engineered Escherichia coli via the salvage pathway through increasing GTP synthesis and β‐galactosidase modification
Sign Up to like & getrecommendations! Published in 2019 at "Biotechnology and Bioengineering"
DOI: 10.1002/bit.27160
Abstract: 3‐Fucosyllactose (3‐FL) is one of the major fucosylated oligosaccharides in human milk. Along with 2′‐fucosyllactose (2′‐FL), it is known for its prebiotic, immunomodulator, neonatal brain development, and antimicrobial function. Whereas the biological production of 2′‐FL… read more here.
Keywords: milk; production; coli bl21; biosynthesis human ... See more keywords
Arsenic transformation mediated by gut microbiota affects the fecundity of Caenorhabditis elegans.
Sign Up to like & getrecommendations! Published in 2020 at "Environmental pollution"
DOI: 10.1016/j.envpol.2020.113991
Abstract: Arsenic biotransformation has been discovered in guts of soil invertebrates. Reproduction of invertebrates is sensitive to arsenic contamination in soils. However, little is known about the impact of gut microbe-mediated arsenic biotransformation on the fecundity… read more here.
Keywords: mediated gut; arsenic transformation; fecundity; coli bl21 ... See more keywords
Cloning, expression and characterization of a thermo-alkali-stable xylanase from Aspergillus oryzae LC1 in Escherichia coli BL21(DE3).
Sign Up to like & getrecommendations! Published in 2019 at "Protein expression and purification"
DOI: 10.1016/j.pep.2019.105551
Abstract: In the present investigation, cloning and overexpression of xylanase (XynF1), the main xylanase of A. oryzae LC1, was performed in prokaryotic system E. coli BL21(DE3) to produce recombinant xylanase with high titer of specific activity… read more here.
Keywords: oryzae lc1; xylanase; bl21 de3; coli bl21 ... See more keywords
Metabolic engineering of Escherichia coli BL21 (DE3) for de novo production of l-DOPA from d-glucose
Sign Up to like & getrecommendations! Published in 2019 at "Microbial Cell Factories"
DOI: 10.1186/s12934-019-1122-0
Abstract: BackgroundProduction of l-tyrosine is gaining grounds as the market size of 3,4-dihydroxyphenyl-l-alanine (l-DOPA) is expected to increase due to increasing cases of Parkinson’s disease a neurodegenerative disease. Attempts to overproduce l-tyrosine for conversion to l-DOPA… read more here.
Keywords: dopa; bl21 de3; coli bl21; production ... See more keywords
Metabolic network reconstruction and phenome analysis of the industrial microbe, Escherichia coli BL21(DE3)
Sign Up to like & getrecommendations! Published in 2018 at "PLoS ONE"
DOI: 10.1371/journal.pone.0204375
Abstract: Escherichia coli BL21(DE3) is an industrial model microbe for the mass-production of bioproducts such as biofuels, biorefineries, and recombinant proteins. However, despite its important role in scientific research and biotechnological applications, a high-quality metabolic network… read more here.
Keywords: model; bl21 de3; coli bl21; metabolic network ... See more keywords
Optimization of Monochloroacetic Acid Biodegradation by Recombinant E. coli BL21 (DE3)/pET-bcfd1 Carrying Haloacid Dehalogenase Gene from Bacillus cereus IndB1
Sign Up to like & getrecommendations! Published in 2021 at "International Journal of Renewable Energy Development"
DOI: 10.14710/ijred.2021.38887
Abstract: In recent years, attention to microbial dehalogenase has continually increased due to its potential application, both in bioremediation and in the biosynthesis of fine chemicals. Many microbial recombinant strains carrying dehalogenase gene have been developed,… read more here.
Keywords: bl21 de3; dehalogenase gene; pet bcfd1; haloacid dehalogenase ... See more keywords
Enhancing the biosynthesis of riboflavin in the recombinant Escherichia coli BL21 strain by metabolic engineering
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2022.1111790
Abstract: In this study, to construct the riboflavin-producing strain R1, five key genes, ribA, ribB, ribC, ribD, and ribE, were cloned and ligated to generate the plasmid pET-AE, which was overexpressed in Escherichia coli BL21. The… read more here.
Keywords: coli bl21; strain; escherichia coli; riboflavin ... See more keywords