Short DNA containing χ sites enhances DNA stability and gene expression in E. coli cell-free transcription-translation systems.
Sign Up to like & getrecommendations! Published in 2017 at "Biotechnology and bioengineering"
DOI: 10.1002/bit.26333
Abstract: Escherichia coli cell-free transcription-translation (TXTL) systems offer versatile platforms for advanced biomanufacturing and for prototyping synthetic biological parts and devices. Production and testing could be accelerated with the use of linear DNA, which can be… read more here.
Keywords: dna; free transcription; cell free; expression ... See more keywords
Producing a glycosylating Escherichia coli cell factory: The placement of the bacterial oligosaccharyl transferase pglB onto the genome.
Sign Up to like & getrecommendations! Published in 2018 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2017.11.023
Abstract: Although Escherichia coli has been engineered to perform N-glycosylation of recombinant proteins, an optimal glycosylating strain has not been created. By inserting a codon optimised Campylobacter oligosaccharyltransferase onto the E. coli chromosome, we created a glycoprotein… read more here.
Keywords: glycosylating escherichia; escherichia coli; cell factory; glycoprotein ... See more keywords
Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System.
Sign Up to like & getrecommendations! Published in 2018 at "Molecular cell"
DOI: 10.1016/j.molcel.2017.12.007
Abstract: CRISPR-Cas systems offer versatile technologies for genome engineering, yet their implementation has been outpaced by ongoing discoveries of new Cas nucleases and anti-CRISPR proteins. Here, we present the use of E. coli cell-free transcription-translation (TXTL) systems… read more here.
Keywords: transcription translation; crispr; free transcription; cell free ... See more keywords
A Methylation-Directed, Synthetic Pap Switch Based on Self-Complementary Regulatory DNA Reconstituted in an All E. coli Cell-Free Expression System.
Sign Up to like & getrecommendations! Published in 2021 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.1c00326
Abstract: Pyelonephritis-associated pili (pap) enable migration of the uropathogenic Escherichia coli strain (UPEC) through the urinary tract. UPEC can switch between a stable 'ON phase' where the corresponding pap genes are expressed and a stable 'OFF… read more here.
Keywords: methylation; phase; regulatory dna; dna ... See more keywords
In-cell NMR as a sensitive tool to monitor physiological condition of Escherichia coli
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-59076-2
Abstract: The in-cell NMR technique offers significant insights into the structure and function of heterologous proteins in the physiological intracellular environment at an atomic resolution. Escherichia coli (E. coli) is one of the most widely used… read more here.
Keywords: resolution; escherichia coli; cell nmr; cell ... See more keywords
Engineered Escherichia coli cell factory for anthranilate over-production
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Microbiology"
DOI: 10.3389/fmicb.2023.1081221
Abstract: Anthranilate is a key platform chemical in high demand for synthesizing food ingredients, dyes, perfumes, crop protection compounds, pharmaceuticals, and plastics. Microbial-based anthranilate production strategies have been developed to overcome the unstable and expensive supply… read more here.
Keywords: cell factory; escherichia coli; production; anthranilate production ... See more keywords