Detergent-free isolation of CYP450-reductase's FMN-binding domain in E. coli lipid-nanodiscs using a charge-free polymer.
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DOI: 10.1039/d1cc07193a
Abstract: The membrane-anchored flavin mononucleotide binding domain (FBD) of CYP450 reductase was extracted in E. coli lipid-nanodiscs using charge-free pentyl-inulin polymer. FBD in nanodiscs was found to be conformationally homogenous and enabled high-resolution NMR probing. 31P… read more here.
Keywords: cyp450 reductase; nanodiscs using; binding domain; coli lipid ... See more keywords