Visual and quantitative detection of E. coli O157:H7 by coupling immunomagnetic separation and quantum dot-based paper strip
Sign Up to like & getrecommendations! Published in 2021 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-021-03395-4
Abstract: Simple and visual quantitative detection of foodborne pathogens can effectively reduce the outbreaks of foodborne diseases. Herein, we developed a simple and sensitive quantum dot (QD)-based paper device for visual and quantitative detection of Escherichia… read more here.
Keywords: detection; paper; visual quantitative; coli o157 ... See more keywords
Proteomic changes of viable but nonculturable (VBNC) Escherichia coli O157:H7 induced by low moisture in an artificial soil
Sign Up to like & getrecommendations! Published in 2020 at "Biology and Fertility of Soils"
DOI: 10.1007/s00374-020-01520-6
Abstract: We studied the viable but nonculturable (VBNC) state of the human pathogen Escherichia coli O157:H7 during desiccation using genomic, proteomic, and direct methods in a model soil under controlled conditions. During desiccation, the bacterial cells… read more here.
Keywords: state; viable nonculturable; coli o157; vbnc ... See more keywords
Rapid detection of E. coli O157:H7 by a novel access with combination of improved sample preparation and real-time PCR
Sign Up to like & getrecommendations! Published in 2020 at "Food Science and Biotechnology"
DOI: 10.1007/s10068-020-00758-y
Abstract: Foodborne bacteria are typically present at very low concentrations in food. This study describes a quick and simple method for concentrating E. coli O157:H7 present in lettuce and cabbage, without microbial enrichment culture. This method… read more here.
Keywords: real time; time pcr; coli o157; method ... See more keywords
Quantification and Discrimination of Viable and Dead Escherichia coli O157:H7 Cells from Chicken Without Enrichment by Ethidium Bromide Monoazide Real-time Loop-Mediated Isothermal Amplification
Sign Up to like & getrecommendations! Published in 2017 at "Food Analytical Methods"
DOI: 10.1007/s12161-017-1092-y
Abstract: In this study, a rapid and sensitive method of real-time loop-mediated isothermal amplification (Rti-LAMP) assays was developed for quantification and discrimination of viable and heat-killed E. coli O157:H7 cells treated with low concentration of ethidium… read more here.
Keywords: real time; coli o157; value; cfu ... See more keywords
Simultaneous detection of Salmonella spp., Pseudomonas aeruginosa, Bacillus cereus, and Escherichia coli O157:H7 in environmental water using PMA combined with mPCR
Sign Up to like & getrecommendations! Published in 2020 at "Journal of Microbiology"
DOI: 10.1007/s12275-020-0084-6
Abstract: A multiplex polymerase chain reaction (mPCR) with propidium monoazide (PMA) and internal amplification control (IAC) for the simultaneous detection of waterborne pathogens Salmonella spp., Pseudomonas aeruginosa, Bacillus cereus, and Escherichia coli O157:H7, was developed. This… read more here.
Keywords: pma; salmonella spp; detection; coli o157 ... See more keywords
Characterization of a novel phage depolymerase specific to Escherichia coli O157:H7 and biofilm control on abiotic surfaces.
Sign Up to like & getrecommendations! Published in 2021 at "Journal of microbiology"
DOI: 10.1007/s12275-021-1413-0
Abstract: The increasing prevalence of foodborne diseases caused by Escherichia coli O157:H7 as well as its ability to form biofilms poses major threats to public health worldwide. With increasing concerns about the limitations of current disinfectant… read more here.
Keywords: escherichia coli; phage; coli o157; coli ... See more keywords
Development of a filtration-based LAMP–LFA method as sensitive and rapid detection of E. coli O157:H7
Sign Up to like & getrecommendations! Published in 2019 at "Journal of Food Science and Technology"
DOI: 10.1007/s13197-019-03740-7
Abstract: Rapid detection of low number of pathogenic bacteria in food is difficult. This study tested the filter-based loop-mediated isothermal amplification-lateral flow immunoassay (LAMP–LFA) method for rapid detection of pathogens in real food. Escherichia coli O157:H7… read more here.
Keywords: rapid detection; detection; coli o157; lamp lfa ... See more keywords
Simultaneous detection of Escherichia coli O157:H7, Staphylococcus aureus and Salmonella by multiplex PCR in milk
Sign Up to like & getrecommendations! Published in 2018 at "3 Biotech"
DOI: 10.1007/s13205-018-1086-5
Abstract: Escherichia coli O157:H7, Staphylococcus aureus, and Salmonella are food-borne pathogens that cause serious gastrointestinal illness and frequent food safety accidents. This study aimed to develop a practical multiplex polymerase chain reaction (mPCR) technique for the… read more here.
Keywords: aureus salmonella; detection; food; coli o157 ... See more keywords
Inactivation of Escherichia coli O157:H7 by ozone in different substrates
Sign Up to like & getrecommendations! Published in 2019 at "Brazilian Journal of Microbiology"
DOI: 10.1007/s42770-018-0025-2
Abstract: Ozone has a broad antimicrobial spectrum and each microorganism species has inherent sensitivity to the gas. The objective of this study was to evaluate the effect of ozone gas on Escherichia coli O157:H7 inoculated on… read more here.
Keywords: escherichia coli; coli; coli o157; ozonated water ... See more keywords
DNA aptamer identification and characterization for E. coli O157 detection using cell based SELEX method.
Sign Up to like & getrecommendations! Published in 2017 at "Analytical biochemistry"
DOI: 10.1016/j.ab.2017.08.005
Abstract: Escherichia coli (E. coli) O157:H7 is a foodborne pathogen that causes symptoms in humans. Its rapid identification should be considered to avoid toxic effects of the pathogen. In this study, systematic evolution of ligands by… read more here.
Keywords: detection; dna; selex method; coli o157 ... See more keywords
Aptamer surface functionalization of microfluidic devices using dendrimers as multi-handled templates and its application in sensitive detections of foodborne pathogenic bacteria.
Sign Up to like & getrecommendations! Published in 2019 at "Analytica chimica acta"
DOI: 10.1016/j.aca.2019.01.035
Abstract: A microfluidic system that incorporates both dendrimers and aptamers to detect E. coli O157:H7 is developed. To achieve this, generation 7-polyamidoamine dendrimers were immobilized onto the detection surfaces of PDMS microfluidic channels; subsequently aptamers against E. coli… read more here.
Keywords: detection; pathogenic bacteria; coli o157; aptamer surface ... See more keywords