In Vitro Characterization of the Colibactin-Activating Peptidase ClbP Enables Design of a Fluorogenic Activity Probe.
Sign Up to like & getrecommendations! Published in 2019 at "ACS chemical biology"
DOI: 10.1021/acschembio.9b00540
Abstract: The gut bacterial genotoxin colibactin is linked to the development of colorectal cancer. In the final stages of colibactin’s biosynthesis, an inactive precursor (precolibactin) undergoes proteolytic cleavage by ClbP, an unusual inner-membrane-bound periplasmic peptidase, to… read more here.
Keywords: activity probe; fluorogenic activity; colibactin; vitro ... See more keywords
The reactivity of an unusual amidase may explain colibactin's DNA cross-linking activity.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of the American Chemical Society"
DOI: 10.1021/jacs.9b02453
Abstract: Certain commensal and pathogenic bacteria produce colibactin, a small molecule genotoxin that causes interstrand cross-links in host cell DNA. Though colibactin alkylates DNA, the molecular basis for cross-link formation is unclear. Here, we report that… read more here.
Keywords: colibactin; dna cross; cross; colibactin dna ... See more keywords
Bacterial warhead targets DNA
Sign Up to like & getrecommendations! Published in 2019 at "Science"
DOI: 10.1126/science.363.6428.703-m
Abstract: Toxins The bacterial toxin colibactin causes double-stranded DNA breaks and is associated with the occurrence of bacterially induced colorectal cancer in humans. However, isolation of colibactin is difficult, and its mode of action is poorly… read more here.
Keywords: colibactin; warhead targets; science; bacterial warhead ... See more keywords
The Colibactin Genotoxin Generates DNA Interstrand Cross-Links in Infected Cells
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DOI: 10.1128/mbio.02393-17
Abstract: ABSTRACT Colibactins are hybrid polyketide-nonribosomal peptides produced by Escherichia coli, Klebsiella pneumoniae, and other Enterobacteriaceae harboring the pks genomic island. These genotoxic metabolites are produced by pks-encoded peptide-polyketide synthases as inactive prodrugs called precolibactins, which… read more here.
Keywords: colibactin; cross links; dna; dna cross ... See more keywords
A Toxic Friend: Genotoxic and Mutagenic Activity of the Probiotic Strain Escherichia coli Nissle 1917
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DOI: 10.1128/msphere.00624-21
Abstract: Nissle 1917 is sold as a probiotic and considered safe even though it has been known since 2006 that it harbors the genes for colibactin synthesis. Colibactin is a potent genotoxin that is now linked… read more here.
Keywords: genotoxin; nissle 1917; colibactin; escherichia coli ... See more keywords
Abstract 5900: Tolerance to colibactin correlates with response to chemotherapeutic agents in colorectal cancer
Sign Up to like & getrecommendations! Published in 2023 at "Cancer Research"
DOI: 10.1158/1538-7445.am2023-5900
Abstract: Introduction: The bacterial genotoxin colibactin is enriched in colorectal cancer (CRC) and promotes the accumulation of mutations that drive tumorigenesis. However, systematic assessment of its impact on DNA damage response is lacking and the effect… read more here.
Keywords: colibactin; colorectal cancer; cell lines; response ... See more keywords
Colibactin possessing E. coli isolates in association with colorectal cancer and their genetic diversity among Pakistani population
Sign Up to like & getrecommendations! Published in 2022 at "PLOS ONE"
DOI: 10.1371/journal.pone.0262662
Abstract: Colorectal cancer (CRC) is the third most prevalent cause of tumorigenesis and several pathogenic bacteria have been correlated with aggressive cases of cancer i.e., genotoxin (colibactin) producing Escherichia coli (E. coli). This study was designed… read more here.
Keywords: clb; colibactin; coli isolates; colorectal cancer ... See more keywords
D-Serine reduces the expression of the cytopathic genotoxin colibactin
Sign Up to like & getrecommendations! Published in 2023 at "Microbial Cell"
DOI: 10.15698/mic2023.03.793
Abstract: Some Escherichia coli strains harbour the pks island, a 54 kb genomic island encoding the biosynthesis genes for a genotoxic compound named colibactin. In eukaryotic cells, colibactin can induce DNA damage, cell cycle arrest and… read more here.
Keywords: pks; colibactin; expression; serine reduces ... See more keywords
Colibactin Contributes to the Hypervirulence of pks+ K1 CC23 Klebsiella pneumoniae in Mouse Meningitis Infections
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DOI: 10.3389/fcimb.2017.00103
Abstract: Klebsiella pneumoniae is the most common pathogen of community-acquired meningitis in Taiwan. However, the lack of a physiologically relevant meningitis model for K. pneumoniae has impeded research into its pathogenesis mechanism. Based on the core… read more here.
Keywords: meningitis; colibactin; pks cc23; pneumoniae ... See more keywords
The microbiome-product colibactin hits unique cellular targets mediating host–microbe interaction
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Pharmacology"
DOI: 10.3389/fphar.2022.958012
Abstract: The human microbiota produces molecules that are evolved to interact with the diverse cellular machinery of both the host and microbes, mediating health and diseases. One of the most puzzling microbiome molecules is colibactin, a… read more here.
Keywords: colibactin hits; colibactin; mediating host; cellular targets ... See more keywords
Shining a Light on Colibactin Biology
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DOI: 10.3390/toxins13050346
Abstract: Colibactin is a secondary metabolite encoded by the pks gene island identified in several Enterobacteriaceae, including some pathogenic Escherichia coli (E. coli) commonly enriched in mucosal tissue collected from patients with inflammatory bowel disease and… read more here.
Keywords: colibactin; colibactin biology; coli; biology ... See more keywords