CRISPR/Cas12a collateral cleavage activity for an ultrasensitive assay of RNase H.
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DOI: 10.1039/d1cc06026k
Abstract: We herein describe an ultrasensitive RNase H assay by utilizing CRISPR/Cas12a collateral cleavage activity. Based on this unique design principle, the RNase H activity was successfully determined down to 0.00024 U mL-1, which is quite… read more here.
Keywords: cas12a collateral; rnase; activity; collateral cleavage ... See more keywords