Single-step colony assay with autoinduction of scFv expression for the screening of antibody libraries.
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DOI: 10.2144/btn-2018-0195
Abstract: We present a simple colony assay method for screening antibody libraries based on autoinduction of antibody fragment expression. This protocol eliminates the need for colony size monitoring and a separate induction step for single-chain Fv… read more here.
Keywords: antibody libraries; antibody; colony assay; expression ... See more keywords