Sense and Nonsense of Elevated Column Temperature in Proteomic Bottom-up LC-MS Analyses.
Sign Up to like & getrecommendations! Published in 2020 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.0c00479
Abstract: Elevated column temperature represents a simple means for improving chromatographic separation of peptides. Here, we demonstrated the advantages of the column temperature in peptide separation using state-of-the-art columns. More importantly, we also determined how temperature… read more here.
Keywords: temperature; elevated column; proteomic bottom; bottom analyses ... See more keywords
Improved Reversed Phase Chromatography of Hydrophilic Peptides from Spatial and Temporal Changes in Column Temperature.
Sign Up to like & getrecommendations! Published in 2017 at "Journal of proteome research"
DOI: 10.1021/acs.jproteome.6b01055
Abstract: Reversed phase chromatography is an established method for peptide separation and frequently coupled to electrospray ionization-mass spectrometry for proteomic analysis. Column temperature is one parameter that influences peptide retention and elution, but it is often… read more here.
Keywords: phase chromatography; reversed phase; temperature; column temperature ... See more keywords
Separation of Four Flavonol Glycosides from Solanum rostratum Dunal Using Solvent Sublation Followed by HSCCC and Low Column Temperature Preparative HPLC.
Sign Up to like & getrecommendations! Published in 2018 at "Journal of chromatographic science"
DOI: 10.1093/chromsci/bmy044
Abstract: Hyperoside, 3'-O-methylquercetin 3-O-β-D-galactopyranoside, astragalin and 3'-O-methylquercetin 3-O-β-D-glucopyranoside from an invasive weed Solanum rostratum Dunal were separated and purified successfully by high-speed counter-current chromatography (HSCCC) with a solvent system composed of n-hexane-ethyl acetate-methanol-water (1:7:1:7, v/v) and… read more here.
Keywords: hplc; solanum rostratum; low column; column temperature ... See more keywords