Orthologous CRISPR-Cas9 enzymes for Combinatorial Genetic Screens
Sign Up to like & getrecommendations! Published in 2018 at "Nature biotechnology"
DOI: 10.1038/nbt.4048
Abstract: Combinatorial genetic screening using CRISPR–Cas9 is a useful approach to uncover redundant genes and to explore complex gene networks. However, current methods suffer from interference between the single-guide RNAs (sgRNAs) and from limited gene targeting… read more here.
Keywords: crispr cas9; orthologous crispr; combinatorial genetic; cas9 enzymes ... See more keywords
A polycistronic transgene design for combinatorial genetic perturbations from a single transcript in Drosophila.
Sign Up to like & getrecommendations! Published in 2023 at "PLoS genetics"
DOI: 10.1371/journal.pgen.1010792
Abstract: Experimental models that capture the genetic complexity of human disease and allow mechanistic explorations of the underlying cell, tissue, and organ interactions are crucial to furthering our understanding of disease biology. Such models require combinatorial… read more here.
Keywords: genetic perturbations; disease; drosophila; design ... See more keywords