Combinatorial metabolic engineering of Escherichia coli for de novo production of 2'-fucosyllactose.
Sign Up to like & getrecommendations! Published in 2022 at "Bioresource technology"
DOI: 10.1016/j.biortech.2022.126949
Abstract: 2'-Fucosyllactose (2'-FL) is a kind of fucosylated lactose of human milk oligosaccharides (HMOs). In this work, Escherichia coli MG1655 was metabolically engineered to increase 2'-FL production. The 2'-FL titer was raised from 0.0118 to 0.8062… read more here.
Keywords: coli novo; engineering escherichia; combinatorial metabolic; escherichia coli ... See more keywords
Combinatorial metabolic engineering using an orthogonal tri-functional CRISPR system
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DOI: 10.1038/s41467-017-01695-x
Abstract: Designing an optimal microbial cell factory often requires overexpression, knock-down, and knock-out of multiple gene targets. Unfortunately, such rewiring of cellular metabolism is often carried out sequentially and with low throughput. Here, we report a… read more here.
Keywords: engineering; crispr; tri functional; metabolic engineering ... See more keywords