Identifying Novel Regulators of Vacuolar Trafficking by Combining Fluorescence Imaging-Based Forward Genetic Screening and In Vitro Pollen Germination.
Sign Up to like & getrecommendations! Published in 2017 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-7262-3_17
Abstract: Subcellular targeting of vacuolar proteins depends on cellular machinery regulating vesicular trafficking. Plant-specific vacuolar trafficking routes have been reported. However, regulators mediating these processes are obscure. By combining a fluorescence imaging-based forward genetic approach and… read more here.
Keywords: vacuolar trafficking; fluorescence imaging; based forward; imaging based ... See more keywords
Combining fluorescence imaging with Hi-C to study 3D genome architecture of the same single cell.
Sign Up to like & getrecommendations! Published in 2018 at "Nature protocols"
DOI: 10.1038/nprot.2018.017
Abstract: Fluorescence imaging and chromosome conformation capture assays such as Hi-C are key tools for studying genome organization. However, traditionally, they have been carried out independently, making integration of the two types of data difficult to… read more here.
Keywords: single cell; fluorescence; fluorescence imaging; imaging study ... See more keywords