The comet assay in Folsomia candida: A suitable approach to assess genotoxicity in collembolans.
Sign Up to like & getrecommendations! Published in 2017 at "Environmental toxicology and chemistry"
DOI: 10.1002/etc.3795
Abstract: The present study shows the comet assay technique being successfully applied for the first time to one of the most widely used soil organisms in standardized ecotoxicological tests, Folsomia candida, providing a step forward in… read more here.
Keywords: comet assay; methodology; folsomia candida; assay folsomia ... See more keywords
Comet Assay for the Detection of Single and Double-Strand DNA Breaks.
Sign Up to like & getrecommendations! Published in 2022 at "Methods in molecular biology"
DOI: 10.1007/978-1-0716-1948-3_18
Abstract: DNA damage is a common event that occurs during the life span of the majority of cells, as a result of aberrant energy metabolism, exposure to radiation, DNA damaging chemicals, and viral infections. Rapid, sensitive,… read more here.
Keywords: dna; single double; double stranded; comet assay ... See more keywords
Cell survival after DNA damage in the comet assay.
Sign Up to like & getrecommendations! Published in 2021 at "Archives of toxicology"
DOI: 10.1007/s00204-021-03164-3
Abstract: The comet assay is widely used in basic research, genotoxicity testing, and human biomonitoring. However, interpretation of the comet assay data might benefit from a better understanding of the future fate of a cell with… read more here.
Keywords: comet assay; dna damage; death; dna ... See more keywords
Plants genotoxicity as pollution bioindicator in Jordan using comet assay
Sign Up to like & getrecommendations! Published in 2017 at "Physiology and Molecular Biology of Plants"
DOI: 10.1007/s12298-017-0491-2
Abstract: This study aimed to assess genotoxicity in wild plants grown in Jordan as a pollution bioindicator. Comet assay was used to evaluate the level of DNA damage in plants collected from different areas in Jordan.… read more here.
Keywords: comet assay; dna damage; pollution; pollution bioindicator ... See more keywords
Carbon black induced DNA damage and conformational changes to mouse hepatocytes and DNA molecule: A combined study using comet assay and multi-spectra methods.
Sign Up to like & getrecommendations! Published in 2019 at "Ecotoxicology and environmental safety"
DOI: 10.1016/j.ecoenv.2018.12.044
Abstract: Carbon black (CB), a carbonaceous nanoparticle, has been widely applied in our daily lives and used as a typical model to study environmental safety and health impacts of airborne particles. Although the potential negative effects… read more here.
Keywords: comet assay; dna; dna molecule; carbon black ... See more keywords
Evaluation of the Genotoxic and Antigenotoxic Potential of Lignin-derivative BP-C2 in the Comet Assay In Vivo.
Sign Up to like & getrecommendations! Published in 2020 at "Environmental research"
DOI: 10.1016/j.envres.2020.110321
Abstract: The genotoxic and antigenotoxic potential of BP-C2, a novel lignin-derived polyphenolic composition with ammonium molybdate, was investigated as a radioprotector/radiomitigator for civic applications and as a medical countermeasure for radiation emergencies. Using the alkaline comet… read more here.
Keywords: comet assay; genotoxic antigenotoxic; antigenotoxic potential; lignin ... See more keywords
Effect of combustion-derived particles on genotoxicity and telomere length: a study on human cells and exposed populations.
Sign Up to like & getrecommendations! Published in 2020 at "Toxicology letters"
DOI: 10.1016/j.toxlet.2020.01.002
Abstract: Particulate matter (PM) from combustion processes has been associated with oxidative stress to DNA, whereas effects related to telomere dysfunction are less investigated. We collected air-borne PM from a passenger cabin of a diesel-propelled train… read more here.
Keywords: a549 cells; comet assay; telomere length; combustion ... See more keywords
Novel approach for the detection of alkylated bases using the enzyme-modified comet assay.
Sign Up to like & getrecommendations! Published in 2020 at "Toxicology letters"
DOI: 10.1016/j.toxlet.2020.04.021
Abstract: The enzyme-modified comet assay is widely used for the detection of oxidized DNA lesions. Here we describe for the first time the use of the human alkyladenine DNA glycosylase (hAAG) for the detection of alkylated… read more here.
Keywords: comet assay; detection alkylated; modified comet; alkylated bases ... See more keywords
Comet assay on one- and two-cell mouse embryos.
Sign Up to like & getrecommendations! Published in 2020 at "Toxicology letters"
DOI: 10.1016/j.toxlet.2020.06.003
Abstract: DNA damage quantified as the comet tail length was assessed using in vitro and in vivo comet assay on one- and two-cell mouse embryos obtained by natural mating. The use of a protocol with three… read more here.
Keywords: comet assay; dna damage; one two; embryos ... See more keywords
Optimization of concentrations and exposure durations of commonly used positive controls in the in vitro alkaline comet assay
Sign Up to like & getrecommendations! Published in 2024 at "Toxicology Research"
DOI: 10.1093/toxres/tfae195
Abstract: Abstract Endogenous and exogenous factors cause DNA damage through chemical changes in the genomic DNA structure. The comet assay is a versatile, rapid, and sensitive method for evaluating DNA integrity at the individual cell level.… read more here.
Keywords: exposure durations; comet assay; commonly used; used positive ... See more keywords
Comet assay for quantification of the increased DNA damage burden in primary human chondrocytes with aging and osteoarthritis
Sign Up to like & getrecommendations! Published in 2022 at "Aging Cell"
DOI: 10.1111/acel.13698
Abstract: It is known that chondrocytes from joints with osteoarthritis (OA) exhibit high levels of DNA damage, but the degree to which chondrocytes accumulate DNA damage during “normal aging” has not been established. The goal of… read more here.
Keywords: increased dna; damage; dna damage; comet assay ... See more keywords