Articles with "competent cells" as a keyword



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The optimization system for preparation of TG1 competent cells and electrotransformation

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Published in 2020 at "MicrobiologyOpen"

DOI: 10.1002/mbo3.1043

Abstract: An efficient electrotransformation system that includes electrocompetent cells is a critical component for the success of large‐scale gene transduction and replication. The conditions of TG1 competent cell preparation and optimal electrotransformation were evaluated by investigating… read more here.

Keywords: system; tg1 competent; electrotransformation; efficiency ... See more keywords
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Induction of competent cells for Agrobacterium tumefaciens-mediated stable transformation of common bean (Phaseolus vulgaris L.)

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Published in 2020 at "PLoS ONE"

DOI: 10.1371/journal.pone.0229909

Abstract: Stable transformation of common bean (Phaseolus vulgaris L.) has been successful, to date, only using biolistic-mediated transformation and shoot regeneration from meristem-containing embryo axes. In this study, using precultured embryo axes, and optimal co-cultivation conditions… read more here.

Keywords: transformation; phaseolus; transformation common; common bean ... See more keywords