The Inoue Method for Preparation and Transformation of Competent Escherichia coli: "Ultracompetent" Cells.
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DOI: 10.1101/pdb.prot101196
Abstract: This protocol differs from other transformation procedures in that the bacterial culture is grown at 18°C rather than the conventional 37°C. Otherwise, the protocol is unremarkable and follows a fairly standard course. Under standard laboratory… read more here.
Keywords: preparation transformation; inoue method; method preparation; transformation ... See more keywords