FRET‐MC: A fluorescence melting competition assay for studying G4 structures in vitro
Sign Up to like & getrecommendations! Published in 2020 at "Biopolymers"
DOI: 10.1002/bip.23415
Abstract: G‐quadruplexes (G4) play crucial roles in biology, analytical chemistry and nanotechnology. The stability of G4 structures is impacted by the number of G‐quartets, the length and positions of loops, flanking motifs, as well as additional… read more here.
Keywords: assay studying; fret fluorescence; melting competition; fluorescence melting ... See more keywords
Detection of biotin with zeptomole sensitivity using recombinant spores and a competition assay
Sign Up to like & getrecommendations! Published in 2020 at "Analytical and Bioanalytical Chemistry"
DOI: 10.1007/s00216-020-02854-8
Abstract: Detection of protein-binding analytes is important for many applications. Currently, various instrument-based techniques are used for detecting protein-binding analytes. However, such techniques have several limitations including high cost and time-consuming sample processing. In order to… read more here.
Keywords: recombinant spores; detection; biotin; using recombinant ... See more keywords
Expediting Antibody Discovery with a Cell and Bead Multiplexed Competition Assay
Sign Up to like & getrecommendations! Published in 2018 at "SLAS Discovery"
DOI: 10.1177/2472555218776308
Abstract: With advances in molecular engineering and humanization, monoclonal antibodies are one of the fastest-growing classes of biopharmaceuticals. During antibody discovery, antibody from hybridoma or primary B-cell supernatants is screened for the desired binding characteristics, and… read more here.
Keywords: antibody discovery; cell bead; antibody; competition assay ... See more keywords