Competitive binding assays for measuring the binding affinity of thyroid-disrupting chemicals for integrin αvβ3.
Sign Up to like & getrecommendations! Published in 2020 at "Chemosphere"
DOI: 10.1016/j.chemosphere.2020.126034
Abstract: Some thyroid-disrupting chemicals (TDCs) affect thyroid function by activating the pathways mediated by a typical thyroid hormone (TH) membrane receptor, integrin αvβ3. The present study introduces improved competitive binding assays for the rapid and sensitive… read more here.
Keywords: binding assay; radioligand competitive; binding; competitive binding ... See more keywords
Competitive Binding Kinetics of Methylmercury-Cysteine with Dissolved Organic Matter: Critical Impacts of Thiols on Adsorption and Uptake of Methylmercury by Algae.
Sign Up to like & getrecommendations! Published in 2024 at "Environmental science & technology"
DOI: 10.1021/acs.est.4c01127
Abstract: Complexes with low-molecular-weight thiols are crucial species of methylmercury (MeHg) excreted by anaerobic Hg-methylating microbes, notably, MeHg-cysteine (MeHg-Cys). As MeHg-Cys diffuses into surface water, it would undergo a ligand exchange process with dissolved organic matter… read more here.
Keywords: adsorption uptake; binding kinetics; competitive binding; mehg ... See more keywords
Beyond Competitive Binding: New Biochemical Insights Challenge Endocrine Disrupting Chemical Screening Paradigms.
Sign Up to like & getrecommendations! Published in 2025 at "Environmental science & technology"
DOI: 10.1021/acs.est.5c07050
Abstract: Endocrine-disrupting chemicals (EDCs) pose significant health risks at environmentally relevant levels. Global screening efforts have predominantly relied on competitive binding assays. Here, using biochemical assays and computational simulations, we identified a crucial but largely overlooked… read more here.
Keywords: new biochemical; beyond competitive; biochemical insights; endocrine disrupting ... See more keywords
Colloidal Hydrogel with Staged Sequestration and Release of Molecules Undergoing Competitive Binding.
Sign Up to like & getrecommendations! Published in 2024 at "ACS nano"
DOI: 10.1021/acsnano.4c09342
Abstract: Competitive binding of distinct molecules in the hydrogel interior can facilitate dynamic exchange between the hydrogel and the surrounding environment. The ability to control the rates of sequestration and release of these molecules would enhance… read more here.
Keywords: release molecules; hydrogel; release; competitive binding ... See more keywords
Prototype Digital Lateral Flow Sensor Using Impact Electrochemistry in a Competitive Binding Assay.
Sign Up to like & getrecommendations! Published in 2022 at "ACS sensors"
DOI: 10.1021/acssensors.2c00728
Abstract: This work demonstrates a lateral flow assay concept on the basis of stochastic-impact electrochemistry. To this end, we first elucidate requirements to employ silver nanoparticles as redox-active labels. Then, we present a prototype that utilizes… read more here.
Keywords: impact electrochemistry; lateral flow; electrochemistry; competitive binding ... See more keywords
Competitive binding of E3 ligases TRIM26 and WWP2 controls SOX2 in glioblastoma
Sign Up to like & getrecommendations! Published in 2021 at "Nature Communications"
DOI: 10.1038/s41467-021-26653-6
Abstract: The pluripotency transcription factor SOX2 is essential for the maintenance of glioblastoma stem cells (GSC), which are thought to underlie tumor growth, treatment resistance, and recurrence. To understand how SOX2 is regulated in GSCs, we… read more here.
Keywords: binding ligases; wwp2; sox2; ligases trim26 ... See more keywords
CLASP-mediated competitive binding in protein condensates directs microtubule growth
Sign Up to like & getrecommendations! Published in 2024 at "Nature Communications"
DOI: 10.1038/s41467-024-50863-3
Abstract: Microtubule organization in cells relies on targeting mechanisms. Cytoplasmic linker proteins (CLIPs) and CLIP-associated proteins (CLASPs) are key regulators of microtubule organization, yet the underlying mechanisms remain elusive. Here, we reveal that the C-terminal domain… read more here.
Keywords: protein condensates; mediated competitive; microtubule growth; competitive binding ... See more keywords
Competitive Binding of Transcription Factors Underlies Flexibility of T Peripheral Helper Cells and T Follicular Helper Cells in SLE.
Sign Up to like & getrecommendations! Published in 2022 at "Rheumatology"
DOI: 10.1093/rheumatology/keac112
Abstract: OBJECTIVE Peripheral helper T (Tph) cells interact with B cells and promote immune responses at sites of ectopic lymphoid structures (ELSs). To assess the characteristics of Tph cells, we investigated the phenotype of T helper… read more here.
Keywords: tph cells; peripheral helper; helper; helper cells ... See more keywords
A Novel Competitive Binding Screening Assay Reveals Sennoside B as a Potent Natural Product Inhibitor of TNF-α
Sign Up to like & getrecommendations! Published in 2021 at "Biomedicines"
DOI: 10.3390/biomedicines9091250
Abstract: Natural products (NPs) have played a significant role in drug discovery for diverse diseases, and numerous attempts have been made to discover promising NP inhibitors of tumor necrosis factor α (TNF-α), a major therapeutic target… read more here.
Keywords: inhibitor; inhibitor tnf; competitive binding; screening assay ... See more keywords