A Single‐Framework Synthetic Antibody Library Containing a Combination of Canonical and Variable Complementarity‐Determining Regions
Sign Up to like & getrecommendations! Published in 2017 at "ChemBioChem"
DOI: 10.1002/cbic.201700279
Abstract: Synthetic antibody libraries have been used to generate antibodies with favorable biophysical and pharmacological properties. Here, we describe the design, construction, and validation of a phage‐displayed antigen‐binding fragment (Fab) library built on a modified trastuzumab… read more here.
Keywords: complementarity determining; framework; antibody; determining regions ... See more keywords
Asparagine Deamidation in a Complementarity Determining Region of a Recombinant Monoclonal Antibody in Complex with Antigen.
Sign Up to like & getrecommendations! Published in 2018 at "Analytical chemistry"
DOI: 10.1021/acs.analchem.8b01322
Abstract: Asparagine deamidation in the complementarity determining regions of recombinant monoclonal antibodies has been extensively studied and shown to have a negative impact on antigen binding. Those asparagine residues are typically exposed and thus have a… read more here.
Keywords: complementarity determining; antibody; recombinant monoclonal; antigen ... See more keywords
Design Strategy to Create Antibody Mimetics Harbouring Immobilised Complementarity Determining Region Peptides for Practical Use
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-57713-4
Abstract: Monoclonal antibodies (mAbs) are attractive therapeutics for treating a wide range of human disorders, and bind to the antigen through their complementarity-determining regions (CDRs). Small stable proteins containing structurally retained CDRs are promising alternatives to… read more here.
Keywords: strategy; antibody mimetics; complementarity determining; create ... See more keywords
Antibody homotypic interactions are encoded by germline light chain complementarity determining region 2
Sign Up to like & getrecommendations! Published in 2022 at "Proceedings of the National Academy of Sciences of the United States of America"
DOI: 10.1073/pnas.2201562119
Abstract: Significance Weak transient interactions are fundamental to immune responses, enabling avidity-driven triggers for pathogen neutralization and cellular regulation. In contrast to obligate binding interactions that can be directly investigated structurally, the low or transitory abundance… read more here.
Keywords: complementarity determining; light chain; homotypic; germline light ... See more keywords
Peptide Aptamer of Complementarity-determining Region to Detect Avian Influenza Virus.
Sign Up to like & getrecommendations! Published in 2019 at "Journal of biomedical nanotechnology"
DOI: 10.1166/jbn.2019.2772
Abstract: Despite significant progress in the development of diagnostic methods for influenza, avian influenza (AI) infection continues to represent a substantial threat to human health. Among the subtypes of AI, H5 influenza is highly infectious to… read more here.
Keywords: avian influenza; complementarity determining; influenza; peptide aptamer ... See more keywords
Complementarity determining regions and frameworks contribute to the disulfide bond independent folding of intrinsically stable scFv
Sign Up to like & getrecommendations! Published in 2017 at "PLoS ONE"
DOI: 10.1371/journal.pone.0189964
Abstract: CyDisCo is a system facilitating disulfide bond formation in recombinant proteins in the cytoplasm of Escherichia coli. Previously we screened for soluble expression of single chain antibody fragments (scFv) in the cytoplasm of E. coli… read more here.
Keywords: complementarity determining; disulfide bond; cydisco; scfv ... See more keywords
In Silico and Structural Analyses Demonstrate That Intrinsic Protein Motions Guide T Cell Receptor Complementarity Determining Region Loop Flexibility
Sign Up to like & getrecommendations! Published in 2018 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2018.00674
Abstract: T-cell immunity is controlled by T cell receptor (TCR) binding to peptide major histocompatibility complexes (pMHCs). The nature of the interaction between these two proteins has been the subject of many investigations because of its… read more here.
Keywords: complementarity determining; tcr; flexibility; cell receptor ... See more keywords
Chickens, more than humans, focus the diversity of their immunoglobulin genes on the complementarity-determining region but utilise amino acids, indicative of a more cross-reactive antibody repertoire
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Immunology"
DOI: 10.3389/fimmu.2022.837246
Abstract: The mechanisms of B-cell diversification differ greatly between aves and mammals, but both produce B cells and antibodies capable of supporting an effective immune response. To see how differences in the generation of diversity might… read more here.
Keywords: immunoglobulin genes; chickens humans; complementarity determining; diversity ... See more keywords
Characterization of Variable Region Genes and Discovery of Key Recognition Sites in the Complementarity Determining Regions of the Anti-Thiacloprid Monoclonal Antibody
Sign Up to like & getrecommendations! Published in 2020 at "International Journal of Molecular Sciences"
DOI: 10.3390/ijms21186857
Abstract: Sequence-defined recombinant antibodies (rAbs) have emerged as alternatives to hybridoma-secreted monoclonal antibodies (mAbs) for performing immunoassays. However, the polyploidy nature of hybridomas often leads to the coexistence of aberrant or non-specific functional variable region (VR)… read more here.
Keywords: complementarity determining; antibody; determining regions; anti thiacloprid ... See more keywords