DNA–RNA complementation on silicon wafer for thyroid cancer determination
Sign Up to like & getrecommendations! Published in 2020 at "Biotechnology and Applied Biochemistry"
DOI: 10.1002/bab.1961
Abstract: One of the current issues with thyroid tumor is early diagnosis as it makes the higher possibility of curing. This research was focused to detect and quantify the level of specific target sequence complementation of… read more here.
Keywords: silicon wafer; dna; target; complementation ... See more keywords
CRISPR‐Mediated Enzyme Fragment Complementation Assay for Quantification of the Stability of Splice Isoforms
Sign Up to like & getrecommendations! Published in 2022 at "ChemBioChem"
DOI: 10.1002/cbic.202200012
Abstract: Small‐molecule splicing modulators exemplified by an FDA‐approved drug, risdiplam, are a new pharmacological modality for regulating the expression and stability of splice isoforms. We report a CRISPR‐mediated enzyme fragment complementation (EFC) assay to quantify the… read more here.
Keywords: complementation; efc assay; stability splice; crispr mediated ... See more keywords
Screening of Interactions with the ESCRT Machinery by a Gaussia princeps Split Luciferase-Based Complementation Assay.
Sign Up to like & getrecommendations! Published in 2019 at "Methods in molecular biology"
DOI: 10.1007/978-1-4939-9492-2_21
Abstract: The endosomal sorting complex required for transport (ESCRT) machinery comprises five complexes that act sequentially to recruit and cluster transmembrane cargo proteins (ESCRT-0), drive membrane curving (ESCRT-I and II), catalyze fission of cargo-containing vesicles (ESCRT-III… read more here.
Keywords: complementation; interactions escrt; escrt machinery; split luciferase ... See more keywords
Severe telomere shortening in Fanconi anemia complementation group L.
Sign Up to like & getrecommendations! Published in 2021 at "Molecular biology reports"
DOI: 10.1007/s11033-020-06101-2
Abstract: Fanconi Anemia (FA) is a rare genetic disease with the incidence of 1 in 360,000 and is characterised by bone marrow failure, physical abnormalities, pancytopenia, and high frequency of chromosomal breakage and increased risk of… read more here.
Keywords: telomere shortening; fanconi anemia; telomere length; severe telomere ... See more keywords
Single-Parent Expression Is a General Mechanism Driving Extensive Complementation of Non-syntenic Genes in Maize Hybrids
Sign Up to like & getrecommendations! Published in 2018 at "Current Biology"
DOI: 10.1016/j.cub.2017.12.027
Abstract: Maize (Zea mays L.) displays an exceptional degree of structural genomic diversity [1, 2]. In addition, variation in gene expression further contributes to the extraordinary phenotypic diversity and plasticity of maize. This study provides a… read more here.
Keywords: single parent; syntenic genes; complementation; non syntenic ... See more keywords
Bioluminescence Goes Dark: Boosting the Performance of Bioluminescent Sensor Proteins Using Complementation Inhibitors
Sign Up to like & getrecommendations! Published in 2022 at "ACS Sensors"
DOI: 10.1021/acssensors.2c01726
Abstract: Bioluminescent sensor proteins have recently gained popularity in both basic research and point-of-care diagnostics. Sensor proteins based on intramolecular complementation of split NanoLuc are particularly attractive because their intrinsic modular design enables for systematic tuning… read more here.
Keywords: performance bioluminescent; complementation; bioluminescent sensor; sensor proteins ... See more keywords
A Tri-part Protein Complementation System Using Antibody-Small Peptide Fusions Enables Homogeneous Immunoassays
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-07569-y
Abstract: Protein-fragment complementation is a valuable tool for monitoring protein interactions. In complementation assays, the reporter fragments are directly fused to the interacting proteins, eliminating the possibility of monitoring native interactions. In principle, complementation could be… read more here.
Keywords: protein; system; antibody; complementation ... See more keywords
Plasmid-based complementation of large deletions in Phaeodactylum tricornutum biosynthetic genes generated by Cas9 editing
Sign Up to like & getrecommendations! Published in 2020 at "Scientific Reports"
DOI: 10.1038/s41598-020-70769-6
Abstract: The model diatom Phaeodactylum tricornutum is an attractive candidate for synthetic biology applications. Development of auxotrophic strains of P. tricornutum would provide alternative selective markers to commonly used antibiotic resistance genes. Here, using CRISPR/Cas9, we… read more here.
Keywords: cas9 editing; complementation; plasmid based; phaeodactylum tricornutum ... See more keywords
Coinfection in the host can result in functional complementation between live vaccines and virulent virus
Sign Up to like & getrecommendations! Published in 2022 at "Virulence"
DOI: 10.1080/21505594.2022.2082645
Abstract: ABSTRACT One of the greatest achievements of the last century is the development of vaccines against viral diseases. Vaccines are essential for battling infectious diseases and many different formulations are available, including live attenuated vaccines.… read more here.
Keywords: vaccines virulent; virulent virus; complementation; functional complementation ... See more keywords
Single-parent expression complementation contributes to phenotypic heterosis in maize hybrids
Sign Up to like & getrecommendations! Published in 2022 at "Plant Physiology"
DOI: 10.1093/plphys/kiac180
Abstract: Abstract The dominance model of heterosis explains the superior performance of F1-hybrids via the complementation of deleterious alleles by beneficial alleles in many genes. Genes active in one parent but inactive in the second lead… read more here.
Keywords: spe; complementation; heterosis; expression ... See more keywords
Xenogeneic chimera—Generated by blastocyst complementation—As a potential unlimited source of recipient‐tailored organs
Sign Up to like & getrecommendations! Published in 2017 at "Xenotransplantation"
DOI: 10.1111/xen.12327
Abstract: Blastocyst complementation refers to the injection of cells into a blastocyst. The technology allows for the creation of chimeric animals, which have the potential to be used as an unlimited source of organ donors. Pluripotent… read more here.
Keywords: unlimited source; chimera; blastocyst complementation; complementation ... See more keywords