Streamlining the Chemoenzymatic Synthesis of Complex N-Glycans by a Stop and Go Strategy
Sign Up to like & getrecommendations! Published in 2018 at "Nature chemistry"
DOI: 10.1038/s41557-018-0188-3
Abstract: Contemporary chemoenzymatic approaches can provide highly complex multi-antennary N-linked glycans. These procedures are, however, very demanding and typically involve as many as 100 chemical steps to prepare advanced intermediates that can be diversified by glycosyltransferases… read more here.
Keywords: complex glycans; streamlining chemoenzymatic; multi antennary; chemoenzymatic synthesis ... See more keywords
On-chip biosynthesis of GM1 pentasaccharide-related complex glycans.
Sign Up to like & getrecommendations! Published in 2018 at "Chemical communications"
DOI: 10.1039/c8cc06526h
Abstract: A functional glycan chip combined with on-chip enzymatic glycosylation was developed to prepare complex glycan sources and to apply glycan-involved applications simultaneously. GM3 trisaccharide, GM2 tetrasaccharide, and GM1 pentasaccharide were successfully directly biosynthesized on lactose-immobilized… read more here.
Keywords: biosynthesis; gm1 pentasaccharide; complex glycans; related complex ... See more keywords
Inactivation of N-Acetylglucosaminyltransferase I and α1,3-Fucosyltransferase Genes in Nicotiana tabacum BY-2 Cells Results in Glycoproteins With Highly Homogeneous, High-Mannose N-Glycans
Sign Up to like & getrecommendations! Published in 2021 at "Frontiers in Plant Science"
DOI: 10.3389/fpls.2021.634023
Abstract: Nicotiana tabacum Bright Yellow-2 (BY-2) suspension cells are among the most commonly used plant cell lines for producing biopharmaceutical glycoproteins. Recombinant glycoproteins are usually produced with a mix of high-mannose and complex N-glycans. However, N-glycan… read more here.
Keywords: high mannose; cell lines; mannose glycans; nicotiana tabacum ... See more keywords