Bottom-up structural proteomics: cryoEM of protein complexes enriched from the cellular milieu
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DOI: 10.1038/s41592-019-0637-y
Abstract: X-ray crystallography often requires non-native constructs involving mutations or truncations, and is challenged by membrane proteins and large multicomponent complexes. We present here a bottom-up endogenous structural proteomics approach whereby near-atomic-resolution cryo electron microscopy (cryoEM)… read more here.
Keywords: protein; cellular milieu; microscopy; complexes enriched ... See more keywords