Analysis of tripartite Synaptotagmin‐1‐SNARE‐complexin‐1 complexes in solution
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DOI: 10.1002/2211-5463.13503
Abstract: Characterizing interactions of Synaptotagmin‐1 with the SNARE complex is crucial to understand the mechanism of neurotransmitter release. X‐ray crystallography revealed how the Synaptotagmin‐1 C2B domain binds to the SNARE complex through a so‐called primary interface… read more here.
Keywords: snare; tripartite interface; complexin; synaptotagmin snare ... See more keywords
Membrane Defects Recruit C-Terminus of Complexin to Synaptic Vesicles
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DOI: 10.1016/j.bpj.2016.11.2138
Abstract: Single vesicle-vesicle fusion assays based on FRET of lipid or content mixing [1-3] are widely used to investigate complexin on vesicle docking [4] and fast fusion process [5]. Most recently, we discovered the important role… read more here.
Keywords: synaptic vesicles; diao; complexin; membrane defects ... See more keywords
Complexin Suppresses Spontaneous Exocytosis by Capturing the Membrane-Proximal Regions of VAMP2 and SNAP25
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DOI: 10.1016/j.celrep.2020.107926
Abstract: Summary The neuronal protein complexin contains multiple domains that exert clamping and facilitatory functions to tune spontaneous and action potential-triggered synaptic release. We address the clamping mechanism and show that the accessory helix of complexin… read more here.
Keywords: membrane proximal; complexin; accessory helix; membrane ... See more keywords
Unique Structural Features of Membrane-Bound C-Terminal Domain Motifs Modulate Complexin Inhibitory Function
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DOI: 10.3389/fnmol.2017.00154
Abstract: Complexin is a small soluble presynaptic protein that interacts with neuronal SNARE proteins in order to regulate synaptic vesicle exocytosis. While the SNARE-binding central helix of complexin is required for both the inhibition of spontaneous… read more here.
Keywords: inhibitory function; terminal domain; membrane bound; complexin ... See more keywords
NSF-mediated disassembly of on- and off-pathway SNARE complexes and inhibition by complexin
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DOI: 10.7554/elife.36497
Abstract: SNARE complex disassembly by the ATPase NSF is essential for neurotransmitter release and other membrane trafficking processes. We developed a single-molecule FRET assay to monitor repeated rounds of NSF-mediated disassembly and reassembly of individual SNARE… read more here.
Keywords: snare; nsf mediated; snare complex; complexin ... See more keywords
Molecular determinants of complexin clamping and activation function
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DOI: 10.7554/elife.71938
Abstract: Previously we reported that Synaptotagmin-1 and Complexin synergistically clamp the SNARE assembly process to generate and maintain a pool of docked vesicles that fuse rapidly and synchronously upon Ca2+ influx (Ramakrishnan et al., 2020). Here,… read more here.
Keywords: activation; molecular determinants; determinants complexin; fusion ... See more keywords