Practical Tips for the Application of HDX-MS to Membrane Proteins, Biomolecular Condensates, and Weak Protein Binders.
Sign Up to like & getrecommendations! Published in 2025 at "Journal of the American Society for Mass Spectrometry"
DOI: 10.1021/jasms.5c00067
Abstract: Hydrogen-deuterium exchange mass spectrometry (HDX-MS) has emerged as a powerful biophysical tool to study protein thermodynamics, dynamics, and function. Despite its increasing use, effective implementation of HDX-MS requires knowledge of protein chemistry, folding, liquid chromatography,… read more here.
Keywords: condensates weak; proteins biomolecular; hdx; biomolecular condensates ... See more keywords
Dynamics of protein condensates in weak-binding regime.
Sign Up to like & getrecommendations! Published in 2022 at "Physical review. E"
DOI: 10.1103/physreve.106.044403
Abstract: Weak complementary interactions between proteins and nucleic acids are the main driving forces of intracellular liquid-liquid phase separation. The sticker-spacer model has emerged as a unifying principle for understanding the phase behavior of these multivalent… read more here.
Keywords: condensates weak; weak binding; binding regime; diffusivity ... See more keywords