A CRISPR/Cas9-engineered mouse carrying a conditional knockout allele for the early growth response-1 transcription factor.
Sign Up to like & getrecommendations! Published in 2023 at "Genesis"
DOI: 10.1002/dvg.23515
Abstract: Early growth response 1 (EGR1) mediates transcriptional programs that are indispensable for cell division, differentiation, and apoptosis in numerous physiologies and pathophysiologies. Whole-body EGR1 knockouts in mice (Egr1KO ) have advanced our understanding of EGR1… read more here.
Keywords: growth response; crispr cas9; early growth; mouse ... See more keywords
Dysregulation of humoral immunity in Foxp3 conditional-knockout mice.
Sign Up to like & getrecommendations! Published in 2019 at "Biochemical and biophysical research communications"
DOI: 10.1016/j.bbrc.2019.04.090
Abstract: Foxp3+ regulatory T cells (Tregs) are crucial for maintaining tolerance to self-antigens and preventing autoimmune diseases. Loss of Foxp3 expression leads to autoimmunity and disrupts humoral immune responses, including hyperproduction of immunoglobulin E (IgE). Elucidation… read more here.
Keywords: foxp3floxr26creert2 mice; humoral immunity; knockout mice; mice ... See more keywords
Conditional Knockout of Ankyrin-G In The Mouse Forebrain Results In Bipolar-Like Behavior
Sign Up to like & getrecommendations! Published in 2017 at "European Neuropsychopharmacology"
DOI: 10.1016/j.euroneuro.2016.09.574
Abstract: Background To date, few animal models of bipolar disorder have been developed, and fewer still are based on genetic loci despite the fact that genome wide association studies (GWAS) have now identified a number of… read more here.
Keywords: social defeat; mice; ankyrin; conditional knockout ... See more keywords
A Convenient Cas9-based Conditional Knockout Strategy for Simultaneously Targeting Multiple Genes in Mouse
Sign Up to like & getrecommendations! Published in 2017 at "Scientific Reports"
DOI: 10.1038/s41598-017-00654-2
Abstract: The most powerful way to probe protein function is to characterize the consequence of its deletion. Compared to conventional gene knockout (KO), conditional knockout (cKO) provides an advanced gene targeting strategy with which gene deletion… read more here.
Keywords: targeting multiple; multiple genes; strategy; conditional knockout ... See more keywords
A conditional knockout rat resource of mitochondrial protein-coding genes via a DdCBE-induced premature stop codon
Sign Up to like & getrecommendations! Published in 2023 at "Science Advances"
DOI: 10.1126/sciadv.adf2695
Abstract: Hundreds of pathogenic variants of mitochondrial DNA (mtDNA) have been reported to cause mitochondrial diseases, which still lack effective treatments. It is a huge challenge to install these mutations one by one. We repurposed the… read more here.
Keywords: rat; stop codon; premature stop; coding genes ... See more keywords
Hippo-Yap Signaling Maintains Sinoatrial Node Homeostasis
Sign Up to like & getrecommendations! Published in 2022 at "Circulation"
DOI: 10.1161/circulationaha.121.058777
Abstract: Background: The sinoatrial node (SAN) functions as the pacemaker of the heart, initiating rhythmic heartbeats. Despite its importance, the SAN is one of the most poorly understood cardiac entities because of its small size and… read more here.
Keywords: sinoatrial node; yap; hippo; hippo signaling ... See more keywords
Multiple Gene Transfer and All-In-One Conditional Knockout Systems in Mouse Embryonic Stem Cells for Analysis of Gene Function
Sign Up to like & getrecommendations! Published in 2022 at "Frontiers in Cell and Developmental Biology"
DOI: 10.3389/fcell.2022.870629
Abstract: Mouse embryonic stem cells (ESCs) are powerful tools for functional analysis of stem cell-related genes; however, complex gene manipulations, such as locus-targeted introduction of multiple genes and conditional gene knockout conditional knockout, are technically difficult.… read more here.
Keywords: embryonic stem; mouse embryonic; stem; gene ... See more keywords
Proteomic profiling of eIF3a conditional knockout mice
Sign Up to like & getrecommendations! Published in 2023 at "Frontiers in Molecular Biosciences"
DOI: 10.3389/fmolb.2023.1160063
Abstract: Eukaryotic translation initiation factor 3 subunit A (eIF3a) is the largest subunit of the eukaryotic translation initiation factor 3 (eIF3). eIF3a plays an integral role in protein biosynthesis, hence impacting the onset, development, and treatment… read more here.
Keywords: signaling pathway; knockout; knockout mice; eif3a conditional ... See more keywords
NaV1.1 is essential for proprioceptive signaling and motor behaviors
Sign Up to like & getrecommendations! Published in 2022 at "eLife"
DOI: 10.7554/elife.79917
Abstract: The voltage-gated sodium channel (NaV), NaV1.1, is well-studied in the central nervous system; conversely, its contribution to peripheral sensory neuron function is more enigmatic. Here, we identify a new role for NaV1.1 in mammalian proprioception.… read more here.
Keywords: nav1 essential; knockout; proprioceptive signaling; motor ... See more keywords