Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid
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DOI: 10.1186/s12866-018-1319-0
Abstract: BackgroundTransposon mutagenesis is highly valuable for bacterial genetic and genomic studies. The transposons are usually delivered into host cells through conjugation or electroporation of a suicide plasmid. However, many bacterial species cannot be efficiently conjugated… read more here.
Keywords: mutagenesis; conditional suicide; transposon mutagenesis; transposon ... See more keywords