Rigor and Reproducibility in Confocal Fluorescence Microscopy
Sign Up to like & getrecommendations! Published in 2019 at "Cytometry Part A"
DOI: 10.1002/cyto.a.23924
Abstract: Fluorescence microscopy can be an excellent tool for making quantitative measurements in cells. For particularly rigorous applications, many choose to use confocal microscopes, which use pinholes to throw away the out-of-focus signal, producing an image… read more here.
Keywords: reproducibility confocal; microscopy; fluorescence microscopy; rigor reproducibility ... See more keywords
Cellular localization of tolyporphins, unusual tetrapyrroles, in a microbial photosynthetic community determined using hyperspectral confocal fluorescence microscopy
Sign Up to like & getrecommendations! Published in 2019 at "Photosynthesis Research"
DOI: 10.1007/s11120-019-00625-w
Abstract: The cyanobacterial culture HT-58-2, composed of a filamentous cyanobacterium and accompanying community bacteria, produces chlorophyll a as well as the tetrapyrrole macrocycles known as tolyporphins. Almost all known tolyporphins (A–M except K) contain a dioxobacteriochlorin… read more here.
Keywords: hyperspectral confocal; localization tolyporphins; microscopy; fluorescence microscopy ... See more keywords
Resolution enhancement of confocal fluorescence microscopy via two illumination beams
Sign Up to like & getrecommendations! Published in 2019 at "Optics and Lasers in Engineering"
DOI: 10.1016/j.optlaseng.2019.05.018
Abstract: Abstract Confocal fluorescence microscopy is an effective imaging technique, but its resolution is limited by the diffraction-limit. Fluorescence emission difference (FED) method is a useful way to improve the resolution of confocal fluorescence microscopy, but… read more here.
Keywords: fluorescence microscopy; confocal fluorescence; microscopy; resolution enhancement ... See more keywords
Highly Multiplexed Confocal Fluorescence Lifetime Microscope Designed for Screening Applications
Sign Up to like & getrecommendations! Published in 2021 at "IEEE Journal of Selected Topics in Quantum Electronics"
DOI: 10.1109/jstqe.2020.2997834
Abstract: Protein-protein interactions can be measured in live cells, at nanometer scale, using Fluorescence Lifetime Imaging Microscopy (FLIM) enabled Forster Resonance Energy Transfer (FRET). There are growing interests in exploring protein-protein interactions in drug discovery applications.… read more here.
Keywords: multiplexed confocal; confocal fluorescence; fluorescence lifetime; protein ... See more keywords