A New Method to Characterize Conformation-Specific Antibody by a Combination of Agarose Native Gel Electrophoresis and Contact Blotting
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DOI: 10.3390/antib11020036
Abstract: In this study, we review the agarose native gel electrophoresis that separates proteins and macromolecular complexes in their native state and transfer of the separated proteins from the agarose gel to membranes by contact blotting… read more here.
Keywords: conformation specific; agarose native; contact blotting; gel ... See more keywords
PEDV Infection Generates Conformation-Specific Antibodies That Can Be Effectively Detected by a Cell-Based ELISA
Sign Up to like & getrecommendations! Published in 2021 at "Viruses"
DOI: 10.3390/v13020303
Abstract: Porcine epidemic diarrhea virus (PEDV) is a coronavirus that causes serious and highly contagious enteric disease in swine worldwide. In this study, we constructed a recombinant baculovirus (S-Bac) expressing full-length spike protein of the virulent… read more here.
Keywords: pedv infection; based elisa; cell based; specific antibodies ... See more keywords