Genetic knockdown of genes that are obscure, conserved and essential using CRISPR interference methods in the fission yeast S. pombe.
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DOI: 10.1242/jcs.261186
Abstract: Characterizing functions of essential genes is challenging, as perturbing them is generally lethal. Conditional gene perturbation, including use of temperature-sensitive mutants, has been widely utilized to reveal functions of essential genes in the fission yeast… read more here.
Keywords: conserved essential; crispr interference; fission yeast; essential genes ... See more keywords