Using overlapâextension PCR technique to fusing genes for constructing recombinant plasmids
Sign Up to like & getrecommendations! Published in 2018 at "Journal of Basic Microbiology"
DOI: 10.1002/jobm.201700455
Abstract: Overlapâextension PCR is a method for splice of gene segments to produce focused fragments for constructing recombinant plasmid, but its complexity limits its application. To simplify the protocol and to improve the effectiveness, we employed… read more here.
Keywords: constructing recombinant; overlap extension; using overlap; extension pcr ... See more keywords