Continuous directed evolution for strain and protein engineering.
Sign Up to like & getrecommendations! Published in 2018 at "Current opinion in biotechnology"
DOI: 10.1016/j.copbio.2017.12.020
Abstract: Continuous directed evolution is the 'while loop' of synthetic biology, autonomous cycles of mutation, selection, and self-replication that can lead to the rapid development of industrially relevant organisms, pathways, or molecules. Although this engineering strategy… read more here.
Keywords: engineering; continuous directed; evolution; strain protein ... See more keywords
Continuous Directed Evolution of a Feedback-Resistant Arabidopsis Arogenate Dehydratase in Plantized Escherichia coli.
Sign Up to like & getrecommendations! Published in 2022 at "ACS synthetic biology"
DOI: 10.1021/acssynbio.2c00511
Abstract: Continuous directed evolution (CDE) is a powerful tool for enzyme engineering due to the depth and scale of evolutionary search that it enables. If suitably controlled and calibrated, CDE could be widely applied in plant… read more here.
Keywords: directed evolution; escherichia coli; arabidopsis arogenate; arogenate dehydratase ... See more keywords
Exploiting spatial dimensions to enable parallelized continuous directed evolution
Sign Up to like & getrecommendations! Published in 2022 at "Molecular Systems Biology"
DOI: 10.15252/msb.202210934
Abstract: Current strategies to improve the throughput of continuous directed evolution technologies often involve complex mechanical fluid‐controlling system or robotic platforms, which limits their popularization and application in general laboratories. Inspired by our previous study on… read more here.
Keywords: spatial dimensions; system; space; continuous directed ... See more keywords