Phage-Assisted Continuous Evolution and Selection of Enzymes for Chemical Synthesis
Sign Up to like & getrecommendations! Published in 2021 at "ACS Central Science"
DOI: 10.1021/acscentsci.1c00811
Abstract: Ligand-dependent biosensors are valuable tools for coupling the intracellular concentrations of small molecules to easily detectable readouts such as absorbance, fluorescence, or cell growth. While ligand-dependent biosensors are widely used for monitoring the production of… read more here.
Keywords: assisted continuous; evolution; phage; selection ... See more keywords
Phage-Assisted Continuous Evolution (PACE): A Guide Focused on Evolving Protein–DNA Interactions
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DOI: 10.1021/acsomega.0c03508
Abstract: The uptake of directed evolution methods is increasing, as these powerful systems can be utilized to develop new biomolecules with altered/novel activities, for example, proteins with new catalytic functions or substrate specificities and nucleic acids… read more here.
Keywords: protein; evolution; dna; pace ... See more keywords
Automated continuous evolution of proteins in vivo.
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DOI: 10.1021/acssynbio.0c00135
Abstract: We present automated continuous evolution (ACE), a platform for the hands-free directed evolution of biomolecules. ACE pairs OrthoRep, a genetic system for continuous targeted mutagenesis of user-selected genes in vivo, with eVOLVER, a scalable and… read more here.
Keywords: evolution; evolution proteins; automated continuous; continuous evolution ... See more keywords
Systematic molecular evolution enables robust biomolecule discovery
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DOI: 10.1038/s41592-021-01348-4
Abstract: Evolution occurs when selective pressures from the environment shape inherited variation over time. Within the laboratory, evolution is commonly used to engineer proteins and RNA, but experimental constraints have limited the ability to reproducibly and… read more here.
Keywords: robotics; continuous evolution; biomolecule; phage ... See more keywords
On the continuous (R)evolution of antibody-based and CAR T cell therapies in multiple myeloma: an early 2022 glance into the future
Sign Up to like & getrecommendations! Published in 2022 at "Expert Opinion on Pharmacotherapy"
DOI: 10.1080/14656566.2022.2101362
Abstract: ABSTRACT Introduction The pace at which the identification of novel therapeutic targets has led to the approval of multiple myeloma (MM) agents during the last two decades is nothing more than spectacular. Nevertheless, MM remains… read more here.
Keywords: car cell; continuous evolution; glance; multiple myeloma ... See more keywords