How bacteria control the CRISPR-Cas arsenal.
Sign Up to like & getrecommendations! Published in 2018 at "Current opinion in microbiology"
DOI: 10.1016/j.mib.2017.11.005
Abstract: CRISPR-Cas systems are adaptive immune systems that protect their hosts from predation by bacteriophages (phages) and parasitism by other mobile genetic elements (MGEs). Given the potent nuclease activity of CRISPR effectors, these enzymes must be… read more here.
Keywords: bacteria control; cas arsenal; crispr cas; control crispr ... See more keywords
Multidimensional chemical control of CRISPR-Cas9.
Sign Up to like & getrecommendations! Published in 2017 at "Nature chemical biology"
DOI: 10.1038/nchembio.2224
Abstract: Cas9-based technologies have transformed genome engineering and the interrogation of genomic functions, but methods to control such technologies across numerous dimensions-including dose, time, specificity, and mutually exclusive modulation of multiple genes-are still lacking. We conferred… read more here.
Keywords: multidimensional chemical; crispr cas9; control; control crispr ... See more keywords
Reversible RNA acylation for control of CRISPR–Cas9 gene editing†
Sign Up to like & getrecommendations! Published in 2019 at "Chemical Science"
DOI: 10.1039/c9sc03639c
Abstract: We report the development of post-transcriptional chemical methods that enable control over CRISPR–Cas9 gene editing activity both in in vitro assays and in living cells. We show that an azide-substituted acyl imidazole reagent (NAI-N3) efficiently… read more here.
Keywords: gene editing; crispr cas9; cas9 gene; control crispr ... See more keywords
Rational guide RNA engineering for small-molecule control of CRISPR/Cas9 and gene editing
Sign Up to like & getrecommendations! Published in 2022 at "Nucleic Acids Research"
DOI: 10.1093/nar/gkac255
Abstract: Abstract It is important to control CRISPR/Cas9 when sufficient editing is obtained. In the current study, rational engineering of guide RNAs (gRNAs) is performed to develop small-molecule-responsive CRISPR/Cas9. For our purpose, the sequence of gRNAs… read more here.
Keywords: cas9; crispr cas9; rna; control crispr ... See more keywords