Thermally controlled intein splicing of engineered DNA polymerases provides a robust and generalizable solution for accurate and sensitive molecular diagnostics.
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DOI: 10.1093/nar/gkad368
Abstract: DNA polymerases are essential for nucleic acid synthesis, cloning, sequencing and molecular diagnostics technologies. Conditional intein splicing is a powerful tool for controlling enzyme reactions. We have engineered a thermal switch into thermostable DNA polymerases from… read more here.
Keywords: polymerase; dna; dna polymerases; molecular diagnostics ... See more keywords