Comparing the performance of conventional PCR, RTQ-PCR, and droplet digital PCR assays in detection of Shigella.
Sign Up to like & getrecommendations! Published in 2020 at "Molecular and cellular probes"
DOI: 10.1016/j.mcp.2020.101531
Abstract: The incidence of foodborne infections caused by Shigella spp. is still very high in every year, which poses a great potential threat to public health. Conventional quantification methods based on culture techniques, biochemical, and serological… read more here.
Keywords: detection; pcr rtq; pcr; shigella ... See more keywords
Establishment of conventional PCR and real-time PCR assays for accurate, rapid and quantitative authentication of four mistletoe species.
Sign Up to like & getrecommendations! Published in 2020 at "Phytochemistry"
DOI: 10.1016/j.phytochem.2020.112400
Abstract: Adulterants in processed food and herbal medicines reduce their safety, quality control, or pharmacological efficacy. Four mistletoe species, including Viscum coloratum, inhabit Korea. Leaves and branches of V. coloratum, defined as edible or medicinal mistletoe… read more here.
Keywords: mistletoe; four mistletoe; conventional pcr; mistletoe species ... See more keywords
Development of Real-Time and Conventional PCR Assays for Identifying Stubby Root Nematode Paratrichodorus allius.
Sign Up to like & getrecommendations! Published in 2017 at "Plant disease"
DOI: 10.1094/pdis-10-16-1431-re
Abstract: Paratrichodorus allius is an important pest on many crops, particularly on potato due to its ability to transmit Tobacco rattle virus causing corky ringspot disease on tubers. Detection and identification of P. allius are important… read more here.
Keywords: real time; pcr; conventional pcr; allius ... See more keywords
Development and Validation of Specific Conventional PCR and Real-Time PCR Assays for Rapid Detection/Identification of C. auris from Yeast Isolates and Clinical Specimens.
Sign Up to like & getrecommendations! Published in 2022 at "Mycoses"
DOI: 10.1111/myc.13504
Abstract: BACKGROUND The accurate occurrence rate of C. auris infections is still not clear, mainly due to defects in detection and identification tools routinely used. In this study, we used conventional PCR and real-time PCR assays… read more here.
Keywords: conventional pcr; identification; clinical specimens; detection identification ... See more keywords
Development of a Conventional RT-PCR Assay for Rapid Detection of Porcine Deltacoronavirus with the Same Detection Limit as a SYBR Green-Based Real-Time RT-PCR Assay
Sign Up to like & getrecommendations! Published in 2018 at "BioMed Research International"
DOI: 10.1155/2018/5035139
Abstract: Porcine deltacoronavirus (PDCoV) is a newly discovered coronavirus, which belongs to the family Coronaviridae. It causes watery diarrhea, vomiting, and dehydration in newborn piglets. A sensitive RT-PCR method is urgently required to detect PDCoV infection.… read more here.
Keywords: detection; real time; time pcr; pcr assay ... See more keywords
Detection of human papillomavirus E6 oncoprotein from urine and vaginal self-collected sample via a novel duplex nanoparticle-assisted polymerase chain reaction assay
Sign Up to like & getrecommendations! Published in 2020 at "Materials Express"
DOI: 10.1166/mex.2020.1835
Abstract: To test a new duplex nanoparticle-assisted polymerase chain reaction (PCR) on human papillomavirus (HPV) E6 oncoprotein detection of HPV16/18 positive samples from vaginal and urine self-sampling specimen. Six specimens from cervical biopsy ranging from CIN1… read more here.
Keywords: detection; self sampling; self; conventional pcr ... See more keywords
Detection of pathogenic Escherichia coli on potentially contaminated beef carcasses using cassette PCR and conventional PCR
Sign Up to like & getrecommendations! Published in 2019 at "BMC Microbiology"
DOI: 10.1186/s12866-019-1541-4
Abstract: BackgroundOver a one year period, swabs of 820 beef carcasses were tested for the presence of Shiga toxin-producing Escherichia coli by performing Polymerase Chain Reaction (PCR) in a novel technology termed “cassette PCR”, in comparison… read more here.
Keywords: pcr; beef carcasses; cassette pcr; conventional pcr ... See more keywords
Comparison five primer sets from different genome region of COVID-19 for detection of virus infection by conventional RT-PCR
Sign Up to like & getrecommendations! Published in 2020 at "Iranian Journal of Microbiology"
DOI: 10.18502/ijm.v12i3.3234
Abstract: Background and Objectives: The new beta-coronavirus, which caused Severe Acute Respiratory Coronavirus-2 Syndrome (SARS-CoV-2), a major respiratory outbreak in Wuhan, China in December 2019, is now prevalent in many countries around the world. Identifying PCR-based… read more here.
Keywords: comparison five; primer sets; conventional pcr; sars cov ... See more keywords
Rapid Identification of Paeoniae Radix and Moutan Radicis Cortex Using a SCAR Marker-Based Conventional PCR Assay
Sign Up to like & getrecommendations! Published in 2022 at "Plants"
DOI: 10.3390/plants11212870
Abstract: Paeoniae Radix is a herbal medicine prepared from the dried roots of Paeonia lactiflora, P. anomala subsp. veitchii, and P. japonica. Although the herbal medicines prepared from these species are morphologically similar, they have different… read more here.
Keywords: conventional pcr; paeoniae radix; identification; radicis cortex ... See more keywords