Exclusive use of digital PCR allows an absolute assay of heat-killed Lactobacilli in foods targeting multiple copies of 16S rDNA
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DOI: 10.1038/s41598-020-69206-5
Abstract: The real-time PCR (qPCR) and digital PCR (dPCR) to amplify a single-copy of house-keeping genes (i.e., hsp60, pheS or tuf) are used for the assay of limited microbial species. In general, with a single-copy gene,… read more here.
Keywords: 16s rdna; heat killed; copies 16s; rdna ... See more keywords