Oligonucleotide Primers with G8AE-Clamp Modifications for RT-qPCR Detection of the Low-Copy dsRNA.
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DOI: 10.1007/978-1-4939-9216-4_18
Abstract: We developed a new technique suitable for improved detection of low-copy dsRNA using modified oligonucleotides as primers in RT-qPCR. Insertion of G8AE-clamp residues into primers significantly improves thermal stability of duplexes with RNA without decrease… read more here.
Keywords: low copy; copy dsrna; detection low;